Abstract
A novel chitosan antibacterial dispersion system was prepared by oleoyl-chitosan (OCS) nanoparticles (OCNP). We further investigated the antimicrobial mode of OCNP against Escherichia coli and Staphylococcus aureus using a combination of approaches, including measurement of the effect of lecithin and phosphate groups, the conformation of membrane protein, internalization of fluorescein isothiocyanate (FITC)-labeled OCS nanoparticles (FITC-OCS nanoparticles) observed under fluorescence microscopy and DNA/RNA binding assay. Results of fluorescence experiments indicated that OCNP influenced the structure of bacterial membranes. The lecithin effect showed that OCNP bound to cytoplasmic membrane phospholipids of S. aureus, and phosphate groups played an important role. Fluorescence microscopy observations demonstrated that the way OCNP entered into bacteria varied against strains. The gel-retardation experiment showed that OCNP bound strongly to DNA/RNA and retarded their migration in the gels in a concentration-dependent manner. These results indicate that OCNP exerts its antibacterial activity by damaging the structures of cell membrane and putative binding to extracellular targets such as phosphate groups or intracellular targets such as DNA and RNA.
| Original language | English |
|---|---|
| Pages (from-to) | 127-133 |
| Number of pages | 7 |
| Journal | International Journal of Food Microbiology |
| Volume | 132 |
| Issue number | 2-3 |
| DOIs | |
| Publication status | Published - 2009 Jun 30 |
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Keywords
- Antibacterial dispersion system
- Fluorescence
- Oleoyl-chitosan nanoparticles
ASJC Scopus subject areas
- Food Science
- Microbiology
- Safety, Risk, Reliability and Quality
Cite this
Oleoyl-chitosan nanoparticles inhibits Escherichia coli and Staphylococcus aureus by damaging the cell membrane and putative binding to extracellular or intracellular targets. / Xing, Ke; Chen, Xi Guang; Liu, Cheng Sheng; Cha, Dong Su; Park, Hyun Jin.
In: International Journal of Food Microbiology, Vol. 132, No. 2-3, 30.06.2009, p. 127-133.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Oleoyl-chitosan nanoparticles inhibits Escherichia coli and Staphylococcus aureus by damaging the cell membrane and putative binding to extracellular or intracellular targets
AU - Xing, Ke
AU - Chen, Xi Guang
AU - Liu, Cheng Sheng
AU - Cha, Dong Su
AU - Park, Hyun Jin
PY - 2009/6/30
Y1 - 2009/6/30
N2 - A novel chitosan antibacterial dispersion system was prepared by oleoyl-chitosan (OCS) nanoparticles (OCNP). We further investigated the antimicrobial mode of OCNP against Escherichia coli and Staphylococcus aureus using a combination of approaches, including measurement of the effect of lecithin and phosphate groups, the conformation of membrane protein, internalization of fluorescein isothiocyanate (FITC)-labeled OCS nanoparticles (FITC-OCS nanoparticles) observed under fluorescence microscopy and DNA/RNA binding assay. Results of fluorescence experiments indicated that OCNP influenced the structure of bacterial membranes. The lecithin effect showed that OCNP bound to cytoplasmic membrane phospholipids of S. aureus, and phosphate groups played an important role. Fluorescence microscopy observations demonstrated that the way OCNP entered into bacteria varied against strains. The gel-retardation experiment showed that OCNP bound strongly to DNA/RNA and retarded their migration in the gels in a concentration-dependent manner. These results indicate that OCNP exerts its antibacterial activity by damaging the structures of cell membrane and putative binding to extracellular targets such as phosphate groups or intracellular targets such as DNA and RNA.
AB - A novel chitosan antibacterial dispersion system was prepared by oleoyl-chitosan (OCS) nanoparticles (OCNP). We further investigated the antimicrobial mode of OCNP against Escherichia coli and Staphylococcus aureus using a combination of approaches, including measurement of the effect of lecithin and phosphate groups, the conformation of membrane protein, internalization of fluorescein isothiocyanate (FITC)-labeled OCS nanoparticles (FITC-OCS nanoparticles) observed under fluorescence microscopy and DNA/RNA binding assay. Results of fluorescence experiments indicated that OCNP influenced the structure of bacterial membranes. The lecithin effect showed that OCNP bound to cytoplasmic membrane phospholipids of S. aureus, and phosphate groups played an important role. Fluorescence microscopy observations demonstrated that the way OCNP entered into bacteria varied against strains. The gel-retardation experiment showed that OCNP bound strongly to DNA/RNA and retarded their migration in the gels in a concentration-dependent manner. These results indicate that OCNP exerts its antibacterial activity by damaging the structures of cell membrane and putative binding to extracellular targets such as phosphate groups or intracellular targets such as DNA and RNA.
KW - Antibacterial dispersion system
KW - Fluorescence
KW - Oleoyl-chitosan nanoparticles
UR - http://www.scopus.com/inward/record.url?scp=65649109036&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=65649109036&partnerID=8YFLogxK
U2 - 10.1016/j.ijfoodmicro.2009.04.013
DO - 10.1016/j.ijfoodmicro.2009.04.013
M3 - Article
C2 - 19439383
AN - SCOPUS:65649109036
VL - 132
SP - 127
EP - 133
JO - International Journal of Food Microbiology
JF - International Journal of Food Microbiology
SN - 0168-1605
IS - 2-3
ER -