Oleoyl-chitosan nanoparticles inhibits Escherichia coli and Staphylococcus aureus by damaging the cell membrane and putative binding to extracellular or intracellular targets

Ke Xing, Xi Guang Chen, Cheng Sheng Liu, Dong Su Cha, Hyun Jin Park

Research output: Contribution to journalArticle

60 Citations (Scopus)

Abstract

A novel chitosan antibacterial dispersion system was prepared by oleoyl-chitosan (OCS) nanoparticles (OCNP). We further investigated the antimicrobial mode of OCNP against Escherichia coli and Staphylococcus aureus using a combination of approaches, including measurement of the effect of lecithin and phosphate groups, the conformation of membrane protein, internalization of fluorescein isothiocyanate (FITC)-labeled OCS nanoparticles (FITC-OCS nanoparticles) observed under fluorescence microscopy and DNA/RNA binding assay. Results of fluorescence experiments indicated that OCNP influenced the structure of bacterial membranes. The lecithin effect showed that OCNP bound to cytoplasmic membrane phospholipids of S. aureus, and phosphate groups played an important role. Fluorescence microscopy observations demonstrated that the way OCNP entered into bacteria varied against strains. The gel-retardation experiment showed that OCNP bound strongly to DNA/RNA and retarded their migration in the gels in a concentration-dependent manner. These results indicate that OCNP exerts its antibacterial activity by damaging the structures of cell membrane and putative binding to extracellular targets such as phosphate groups or intracellular targets such as DNA and RNA.

Original languageEnglish
Pages (from-to)127-133
Number of pages7
JournalInternational Journal of Food Microbiology
Volume132
Issue number2-3
DOIs
Publication statusPublished - 2009 Jun 30

Fingerprint

Chitosan
nanoparticles
Cell membranes
chitosan
Nanoparticles
Escherichia coli
cell membranes
Staphylococcus aureus
Cell Membrane
RNA
Lecithin
Phosphates
DNA
Lecithins
Fluorescence microscopy
isothiocyanates
phosphates
fluorescein
fluorescence microscopy
Fluorescein

Keywords

  • Antibacterial dispersion system
  • Fluorescence
  • Oleoyl-chitosan nanoparticles

ASJC Scopus subject areas

  • Food Science
  • Microbiology
  • Safety, Risk, Reliability and Quality

Cite this

Oleoyl-chitosan nanoparticles inhibits Escherichia coli and Staphylococcus aureus by damaging the cell membrane and putative binding to extracellular or intracellular targets. / Xing, Ke; Chen, Xi Guang; Liu, Cheng Sheng; Cha, Dong Su; Park, Hyun Jin.

In: International Journal of Food Microbiology, Vol. 132, No. 2-3, 30.06.2009, p. 127-133.

Research output: Contribution to journalArticle

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AU - Cha, Dong Su

AU - Park, Hyun Jin

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