Oligonol promotes anti-aging pathways via modulation of SIRT1-AMPK-autophagy pathway

Seul Ki Park, Rak Kyun Seong, Ji Ae Kim, Seok Jun Son, Younghoon Kim, Takako Yokozawa, Ok Shin

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

BACKGROUND/OBJECTIVES: Oligonol, mainly found in lychee fruit, is an antioxidant polyphenolic compound which has been shown to have anti-inflammatory and anti-cancer properties. The detailed mechanisms by which oligonol may act as an anti-aging molecule have not been determined. MATERIALS/METHODS: In this study, we evaluated the ability of oligonol to modulate sirtuin (SIRT) expression in human lung epithelial (A549) cells. Oligonol was added to A549 cells and reactive oxygen species production, mitochondrial superoxide formation, and p21 protein levels were measured. Signaling pathways activated upon oligonol treatment were also determined by western blotting. Furthermore, the anti-aging effect of oligonol was evaluated ex vivo in mouse splenocytes and in vivo in Caenorhabditis elegans. RESULTS: Oligonol specifically induced the expression of SIRT1, whose activity is linked to gene expression, metabolic control, and healthy aging. In response to influenza virus infection of A549 cells, oligonol treatment significantly up-regulated SIRT1 expression and down-regulated viral hemagglutinin expression. Oligonol treatment also resulted in the activation of autophagy pathways and the phosphorylation of AMP-activated protein kinase (AMPK). Furthermore, oligonol-treated spleen lymphocytes from old mice showed increased cell proliferation, and mRNA levels of SIRT1 in the lungs of old mice were significantly lower than those in the lungs of young mice. Additionally, in vivo lethality assay revealed that oligonol extended the lifespan of C. elegans infected with lethal Vibrio cholerae. CONCLUSIONS: These data demonstrated that oligonol may act as an anti-aging molecule by modulating SIRT1/autophagy/AMPK pathways.

Original languageEnglish
Pages (from-to)3-10
Number of pages8
JournalNutrition Research and Practice
Volume10
Issue number1
DOIs
Publication statusPublished - 2016 Feb 1

Fingerprint

AMP-activated protein kinase
AMP-Activated Protein Kinases
autophagy
Autophagy
lungs
mice
Caenorhabditis elegans
anti-aging properties
Vibrio cholerae
hemagglutinins
Orthomyxoviridae
splenocytes
lethal genes
superoxide anion
reactive oxygen species
cell proliferation
phosphorylation
polyphenols
spleen
Western blotting

Keywords

  • Aging
  • Oligonol
  • Senescence
  • SIRT1

ASJC Scopus subject areas

  • Nutrition and Dietetics
  • Food Science

Cite this

Oligonol promotes anti-aging pathways via modulation of SIRT1-AMPK-autophagy pathway. / Park, Seul Ki; Seong, Rak Kyun; Kim, Ji Ae; Son, Seok Jun; Kim, Younghoon; Yokozawa, Takako; Shin, Ok.

In: Nutrition Research and Practice, Vol. 10, No. 1, 01.02.2016, p. 3-10.

Research output: Contribution to journalArticle

Park, Seul Ki ; Seong, Rak Kyun ; Kim, Ji Ae ; Son, Seok Jun ; Kim, Younghoon ; Yokozawa, Takako ; Shin, Ok. / Oligonol promotes anti-aging pathways via modulation of SIRT1-AMPK-autophagy pathway. In: Nutrition Research and Practice. 2016 ; Vol. 10, No. 1. pp. 3-10.
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