On-demand three-dimensional freeform fabrication of multi-layered hydrogel scaffold with fluidic channels

Wonhye Lee, Vivian Lee, Samuel Polio, Phillip Keegan, Jong-Hwan Lee, Krisztina Fischer, Je Kyun Park, Seung Schik Yoo

Research output: Contribution to journalArticle

161 Citations (Scopus)

Abstract

One of the challenges in tissue engineering is to provide adequate supplies of oxygen and nutrients to cells within the engineered tissue construct. Soft-lithographic techniques have allowed the generation of hydrogel scaffolds containing a network of fluidic channels, but at the cost of complicated and often time-consuming manufacturing steps. We report a three-dimensional (3D) direct printing technique to construct hydrogel scaffolds containing fluidic channels. Cells can also be printed on to and embedded in the scaffold with this technique. Collagen hydrogel precursor was printed and subsequently crosslinked via nebulized sodium bicarbonate solution. A heated gelatin solution, which served as a sacrificial element for the fluidic channels, was printed between the collagen layers. The process was repeated layer-by-layer to form a 3D hydrogel block. The printed hydrogel block was heated to 37°C, which allowed the gelatin to be selectively liquefied and drained, generating a hollow channel within the collagen scaffold. The dermal fibroblasts grown in a scaffold containing fluidic channels showed significantly elevated cell viability compared to the ones without any channels. The on-demand capability to print fluidic channel structures and cells in a 3D hydrogel scaffold offers flexibility in generating perfusable 3D artificial tissue composites.

Original languageEnglish
Pages (from-to)1178-1186
Number of pages9
JournalBiotechnology and Bioengineering
Volume105
Issue number6
DOIs
Publication statusPublished - 2010 Apr 15
Externally publishedYes

Fingerprint

Hydrogel
Fluidics
Hydrogels
Scaffolds
Fabrication
Scaffolds (biology)
Collagen
Gelatin
Tissue
Sodium bicarbonate
Sodium Bicarbonate
Fibroblasts
Tissue Engineering
Tissue engineering
Nutrients
Printing
Cell Survival
Cells
Oxygen
Food

Keywords

  • 3D freeform fabrication
  • Collagen
  • Gelatin
  • Hydrogel scaffold
  • Perfusion
  • Tissue engineering

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

Cite this

On-demand three-dimensional freeform fabrication of multi-layered hydrogel scaffold with fluidic channels. / Lee, Wonhye; Lee, Vivian; Polio, Samuel; Keegan, Phillip; Lee, Jong-Hwan; Fischer, Krisztina; Park, Je Kyun; Yoo, Seung Schik.

In: Biotechnology and Bioengineering, Vol. 105, No. 6, 15.04.2010, p. 1178-1186.

Research output: Contribution to journalArticle

Lee, Wonhye ; Lee, Vivian ; Polio, Samuel ; Keegan, Phillip ; Lee, Jong-Hwan ; Fischer, Krisztina ; Park, Je Kyun ; Yoo, Seung Schik. / On-demand three-dimensional freeform fabrication of multi-layered hydrogel scaffold with fluidic channels. In: Biotechnology and Bioengineering. 2010 ; Vol. 105, No. 6. pp. 1178-1186.
@article{f4eb71986cec448e97803a1cafd39ae0,
title = "On-demand three-dimensional freeform fabrication of multi-layered hydrogel scaffold with fluidic channels",
abstract = "One of the challenges in tissue engineering is to provide adequate supplies of oxygen and nutrients to cells within the engineered tissue construct. Soft-lithographic techniques have allowed the generation of hydrogel scaffolds containing a network of fluidic channels, but at the cost of complicated and often time-consuming manufacturing steps. We report a three-dimensional (3D) direct printing technique to construct hydrogel scaffolds containing fluidic channels. Cells can also be printed on to and embedded in the scaffold with this technique. Collagen hydrogel precursor was printed and subsequently crosslinked via nebulized sodium bicarbonate solution. A heated gelatin solution, which served as a sacrificial element for the fluidic channels, was printed between the collagen layers. The process was repeated layer-by-layer to form a 3D hydrogel block. The printed hydrogel block was heated to 37°C, which allowed the gelatin to be selectively liquefied and drained, generating a hollow channel within the collagen scaffold. The dermal fibroblasts grown in a scaffold containing fluidic channels showed significantly elevated cell viability compared to the ones without any channels. The on-demand capability to print fluidic channel structures and cells in a 3D hydrogel scaffold offers flexibility in generating perfusable 3D artificial tissue composites.",
keywords = "3D freeform fabrication, Collagen, Gelatin, Hydrogel scaffold, Perfusion, Tissue engineering",
author = "Wonhye Lee and Vivian Lee and Samuel Polio and Phillip Keegan and Jong-Hwan Lee and Krisztina Fischer and Park, {Je Kyun} and Yoo, {Seung Schik}",
year = "2010",
month = "4",
day = "15",
doi = "10.1002/bit.22613",
language = "English",
volume = "105",
pages = "1178--1186",
journal = "Biotechnology and Bioengineering",
issn = "0006-3592",
publisher = "Wiley-VCH Verlag",
number = "6",

}

TY - JOUR

T1 - On-demand three-dimensional freeform fabrication of multi-layered hydrogel scaffold with fluidic channels

AU - Lee, Wonhye

AU - Lee, Vivian

AU - Polio, Samuel

AU - Keegan, Phillip

AU - Lee, Jong-Hwan

AU - Fischer, Krisztina

AU - Park, Je Kyun

AU - Yoo, Seung Schik

PY - 2010/4/15

Y1 - 2010/4/15

N2 - One of the challenges in tissue engineering is to provide adequate supplies of oxygen and nutrients to cells within the engineered tissue construct. Soft-lithographic techniques have allowed the generation of hydrogel scaffolds containing a network of fluidic channels, but at the cost of complicated and often time-consuming manufacturing steps. We report a three-dimensional (3D) direct printing technique to construct hydrogel scaffolds containing fluidic channels. Cells can also be printed on to and embedded in the scaffold with this technique. Collagen hydrogel precursor was printed and subsequently crosslinked via nebulized sodium bicarbonate solution. A heated gelatin solution, which served as a sacrificial element for the fluidic channels, was printed between the collagen layers. The process was repeated layer-by-layer to form a 3D hydrogel block. The printed hydrogel block was heated to 37°C, which allowed the gelatin to be selectively liquefied and drained, generating a hollow channel within the collagen scaffold. The dermal fibroblasts grown in a scaffold containing fluidic channels showed significantly elevated cell viability compared to the ones without any channels. The on-demand capability to print fluidic channel structures and cells in a 3D hydrogel scaffold offers flexibility in generating perfusable 3D artificial tissue composites.

AB - One of the challenges in tissue engineering is to provide adequate supplies of oxygen and nutrients to cells within the engineered tissue construct. Soft-lithographic techniques have allowed the generation of hydrogel scaffolds containing a network of fluidic channels, but at the cost of complicated and often time-consuming manufacturing steps. We report a three-dimensional (3D) direct printing technique to construct hydrogel scaffolds containing fluidic channels. Cells can also be printed on to and embedded in the scaffold with this technique. Collagen hydrogel precursor was printed and subsequently crosslinked via nebulized sodium bicarbonate solution. A heated gelatin solution, which served as a sacrificial element for the fluidic channels, was printed between the collagen layers. The process was repeated layer-by-layer to form a 3D hydrogel block. The printed hydrogel block was heated to 37°C, which allowed the gelatin to be selectively liquefied and drained, generating a hollow channel within the collagen scaffold. The dermal fibroblasts grown in a scaffold containing fluidic channels showed significantly elevated cell viability compared to the ones without any channels. The on-demand capability to print fluidic channel structures and cells in a 3D hydrogel scaffold offers flexibility in generating perfusable 3D artificial tissue composites.

KW - 3D freeform fabrication

KW - Collagen

KW - Gelatin

KW - Hydrogel scaffold

KW - Perfusion

KW - Tissue engineering

UR - http://www.scopus.com/inward/record.url?scp=77951604536&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77951604536&partnerID=8YFLogxK

U2 - 10.1002/bit.22613

DO - 10.1002/bit.22613

M3 - Article

C2 - 19953677

AN - SCOPUS:77951604536

VL - 105

SP - 1178

EP - 1186

JO - Biotechnology and Bioengineering

JF - Biotechnology and Bioengineering

SN - 0006-3592

IS - 6

ER -