One-pot enzymatic conversion of carbon dioxide and utilization for improved microbial growth

Sung Gil Hong, Hancheol Jeon, Han Sol Kim, Seung Hyun Jun, Eonseon Jin, Jungbae Kim

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

We developed a process for one-pot CO<inf>2</inf> conversion and utilization based on simple conversion of CO<inf>2</inf> to bicarbonate at ambient temperature with no energy input, by using the cross-linking-based composites of carboxylated polyaniline nanofibers (cPANFs) and carbonic anhydrase. Carbonic anhydrase was immobilized on cPANFs via the approach of magnetically separable enzyme precipitate coatings (Mag-EPC), which consists of covalent enzyme attachment, enzyme precipitation, and cross-linking with amine-functionalized magnetic nanoparticles. Mag-EPC showed a half-life of 236 days under shaking, even resistance to 70% ethanol sterilization, and recyclability via facile magnetic separation. For one-pot CO<inf>2</inf> conversion and utilization, Mag-EPC was used to accelerate the growth of microalga by supplying bicarbonate from CO<inf>2</inf>, representing 1.8-fold increase of cell concentration when compared to the control sample. After two repeated uses via simple magnetic separation, the cell concentration with Mag-EPC was maintained as high as the first cycle. This one-pot CO<inf>2</inf> conversion and utilization is an alternative as well as complementary process to adsorption-based CO<inf>2</inf> capture and storage as an environmentally friendly approach, demanding no energy input based on the effective action of the stabilized enzyme system.

Original languageEnglish
Pages (from-to)4466-4472
Number of pages7
JournalEnvironmental Science and Technology
Volume49
Issue number7
DOIs
Publication statusPublished - 2015 Apr 7

Fingerprint

Carbon Dioxide
carbon dioxide
enzyme
Enzymes
Growth
Precipitates
coating
Nanofibers
Coatings
Magnetic separation
Carbonic Anhydrases
Bicarbonates
bicarbonate
microalga
Cell Separation
half life
Nanoparticles
Adsorption
Amines
Half-Life

ASJC Scopus subject areas

  • Chemistry(all)
  • Environmental Chemistry

Cite this

One-pot enzymatic conversion of carbon dioxide and utilization for improved microbial growth. / Hong, Sung Gil; Jeon, Hancheol; Kim, Han Sol; Jun, Seung Hyun; Jin, Eonseon; Kim, Jungbae.

In: Environmental Science and Technology, Vol. 49, No. 7, 07.04.2015, p. 4466-4472.

Research output: Contribution to journalArticle

Hong, Sung Gil ; Jeon, Hancheol ; Kim, Han Sol ; Jun, Seung Hyun ; Jin, Eonseon ; Kim, Jungbae. / One-pot enzymatic conversion of carbon dioxide and utilization for improved microbial growth. In: Environmental Science and Technology. 2015 ; Vol. 49, No. 7. pp. 4466-4472.
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