Abstract
We developed a process for one-pot CO<inf>2</inf> conversion and utilization based on simple conversion of CO<inf>2</inf> to bicarbonate at ambient temperature with no energy input, by using the cross-linking-based composites of carboxylated polyaniline nanofibers (cPANFs) and carbonic anhydrase. Carbonic anhydrase was immobilized on cPANFs via the approach of magnetically separable enzyme precipitate coatings (Mag-EPC), which consists of covalent enzyme attachment, enzyme precipitation, and cross-linking with amine-functionalized magnetic nanoparticles. Mag-EPC showed a half-life of 236 days under shaking, even resistance to 70% ethanol sterilization, and recyclability via facile magnetic separation. For one-pot CO<inf>2</inf> conversion and utilization, Mag-EPC was used to accelerate the growth of microalga by supplying bicarbonate from CO<inf>2</inf>, representing 1.8-fold increase of cell concentration when compared to the control sample. After two repeated uses via simple magnetic separation, the cell concentration with Mag-EPC was maintained as high as the first cycle. This one-pot CO<inf>2</inf> conversion and utilization is an alternative as well as complementary process to adsorption-based CO<inf>2</inf> capture and storage as an environmentally friendly approach, demanding no energy input based on the effective action of the stabilized enzyme system.
| Original language | English |
|---|---|
| Pages (from-to) | 4466-4472 |
| Number of pages | 7 |
| Journal | Environmental Science and Technology |
| Volume | 49 |
| Issue number | 7 |
| DOIs | |
| Publication status | Published - 2015 Apr 7 |
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ASJC Scopus subject areas
- Chemistry(all)
- Environmental Chemistry
Cite this
One-pot enzymatic conversion of carbon dioxide and utilization for improved microbial growth. / Hong, Sung Gil; Jeon, Hancheol; Kim, Han Sol; Jun, Seung Hyun; Jin, Eonseon; Kim, Jungbae.
In: Environmental Science and Technology, Vol. 49, No. 7, 07.04.2015, p. 4466-4472.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - One-pot enzymatic conversion of carbon dioxide and utilization for improved microbial growth
AU - Hong, Sung Gil
AU - Jeon, Hancheol
AU - Kim, Han Sol
AU - Jun, Seung Hyun
AU - Jin, Eonseon
AU - Kim, Jungbae
PY - 2015/4/7
Y1 - 2015/4/7
N2 - We developed a process for one-pot CO2 conversion and utilization based on simple conversion of CO2 to bicarbonate at ambient temperature with no energy input, by using the cross-linking-based composites of carboxylated polyaniline nanofibers (cPANFs) and carbonic anhydrase. Carbonic anhydrase was immobilized on cPANFs via the approach of magnetically separable enzyme precipitate coatings (Mag-EPC), which consists of covalent enzyme attachment, enzyme precipitation, and cross-linking with amine-functionalized magnetic nanoparticles. Mag-EPC showed a half-life of 236 days under shaking, even resistance to 70% ethanol sterilization, and recyclability via facile magnetic separation. For one-pot CO2 conversion and utilization, Mag-EPC was used to accelerate the growth of microalga by supplying bicarbonate from CO2, representing 1.8-fold increase of cell concentration when compared to the control sample. After two repeated uses via simple magnetic separation, the cell concentration with Mag-EPC was maintained as high as the first cycle. This one-pot CO2 conversion and utilization is an alternative as well as complementary process to adsorption-based CO2 capture and storage as an environmentally friendly approach, demanding no energy input based on the effective action of the stabilized enzyme system.
AB - We developed a process for one-pot CO2 conversion and utilization based on simple conversion of CO2 to bicarbonate at ambient temperature with no energy input, by using the cross-linking-based composites of carboxylated polyaniline nanofibers (cPANFs) and carbonic anhydrase. Carbonic anhydrase was immobilized on cPANFs via the approach of magnetically separable enzyme precipitate coatings (Mag-EPC), which consists of covalent enzyme attachment, enzyme precipitation, and cross-linking with amine-functionalized magnetic nanoparticles. Mag-EPC showed a half-life of 236 days under shaking, even resistance to 70% ethanol sterilization, and recyclability via facile magnetic separation. For one-pot CO2 conversion and utilization, Mag-EPC was used to accelerate the growth of microalga by supplying bicarbonate from CO2, representing 1.8-fold increase of cell concentration when compared to the control sample. After two repeated uses via simple magnetic separation, the cell concentration with Mag-EPC was maintained as high as the first cycle. This one-pot CO2 conversion and utilization is an alternative as well as complementary process to adsorption-based CO2 capture and storage as an environmentally friendly approach, demanding no energy input based on the effective action of the stabilized enzyme system.
UR - http://www.scopus.com/inward/record.url?scp=84926431933&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84926431933&partnerID=8YFLogxK
U2 - 10.1021/es505143f
DO - 10.1021/es505143f
M3 - Article
C2 - 25815899
AN - SCOPUS:84926431933
VL - 49
SP - 4466
EP - 4472
JO - Environmental Science and Technology
JF - Environmental Science and Technology
SN - 0013-936X
IS - 7
ER -