One-stop microfiber spinning and fabrication of a fibrous cell-encapsulated scaffold on a single microfluidic platform

D. Y. Park, C. H. Mun, E. Kang, D. Y. No, J. Ju, Sang Hoon Lee

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

This paper provides a method for microscale fiber spinning and the in situ construction of a 3D fibrous scaffold on a single microfluidic platform. This platform was also used to fabricate a variety of fibrous scaffolds with diverse compositions without the use of complicated devices. We explored the potential utility of the fibrous scaffolds for tissue engineering applications by constructing a fibrous scaffold encapsulating primary hepatocytes. The cells in scaffold were cultured over seven days and maintained higher viability comparing with 3D alginate non-fibrous block. The main advantage of this platform is that the fibrous structure used to form a scaffold can be generated without damaging the mechanically weak alginate fibers or encapsulated cells because all procedures are performed in a single platform without the intervention of the operator. In addition, the proposed fibrous scaffold permitted high diffusion capability of molecules, which enabled better viability of encapsulated cells than non-fibrous scaffold even in massive cell culture.

Original languageEnglish
Article number024108
JournalBiofabrication
Volume6
Issue number2
DOIs
Publication statusPublished - 2014

Fingerprint

Microfluidics
Scaffolds
Fabrication
Scaffolds (biology)
Tissue Engineering
Alginate
Hepatocytes
Cell Survival
Cell Culture Techniques
Equipment and Supplies
Forms (concrete)
Fibers
Bioelectric potentials
Tissue engineering
Cell culture
alginic acid
Molecules
Chemical analysis

Keywords

  • 3D alginate fibrous scaffold
  • cell-laden fibers
  • microfluidic
  • porosity

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Biomaterials
  • Bioengineering
  • Biomedical Engineering

Cite this

One-stop microfiber spinning and fabrication of a fibrous cell-encapsulated scaffold on a single microfluidic platform. / Park, D. Y.; Mun, C. H.; Kang, E.; No, D. Y.; Ju, J.; Lee, Sang Hoon.

In: Biofabrication, Vol. 6, No. 2, 024108, 2014.

Research output: Contribution to journalArticle

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