Open reading frame 5 protein of porcine circovirus type 2 induces RNF128 (GRAIL) which inhibits mRNA transcription of interferon-β in porcine epithelial cells

Seok Jin Kang, In Byung Park, Taehoon Chun

Research output: Contribution to journalArticlepeer-review

Abstract

A previous study has indicated that mRNA transcript of Rnf128 (Grail) is significantly increased in porcine epithelial cells expressing porcine circovirus type 2 (PCV2) open reading frame 5 (ORF5). RNF128 is an E3 ubiquitin ligase that can modulate the activity of target protein via ubiquitination of specific lysine residues. However, the function of RNF128 in PCV2-infected epithelial cells has not been well studied yet. Thus, the objective of the present study was to examine the functional role of RNF128 in porcine epithelial cells (PK15 cells) after PCV2 infection. Results clearly indicated that PCV2 ORF5 increased the expression of RNF128 which inhibited type I IFN production and enhanced viral replication of PCV2 in PK15 cells. Therefore, up-regulating RNF128 by PCV2 ORF5 can help PCV2 circumvent initial immune surveillance of porcine epithelial cells.

Original languageEnglish
Pages (from-to)79-82
Number of pages4
JournalResearch in Veterinary Science
Volume140
DOIs
Publication statusPublished - 2021 Nov

Keywords

  • ORF5
  • PCV2
  • Porcine epithelial cell
  • RNF128
  • Type I IFN

ASJC Scopus subject areas

  • veterinary(all)

Fingerprint

Dive into the research topics of 'Open reading frame 5 protein of porcine circovirus type 2 induces RNF128 (GRAIL) which inhibits mRNA transcription of interferon-β in porcine epithelial cells'. Together they form a unique fingerprint.

Cite this