TY - JOUR
T1 - Ophiostomatoid fungi associated with the northern spruce engraver, Ips perturbatus, in western Canada
AU - Massoumi Alamouti, Sepideh
AU - Kim, Jae Jin
AU - Humble, Leland M.
AU - Uzunovic, Adnan
AU - Breuil, Colette
N1 - Funding Information:
Acknowledgements This work was supported by the Natural Sciences and Engineering Research Council of Canada. We would like to thank Mr. Rod Garbutt (Natural Resources Canada, Pacific Forestry Centre, Victoria, BC) and Mike Jull (Aleza Lake Research Forest Society, Prince George, BC) for their help with the field works. We also thank Drs. K. Harrison, J. Reid and G. Hausner for providing cultures.
PY - 2007/1
Y1 - 2007/1
N2 - A number of ophiostomatoid fungi were isolated from the spruce-infesting bark beetle, Ips perturbatus and its galleries collected from felled spruce trees and logs in northern BC and the Yukon Territory. Isolates were identified to species using morphological characteristics, nuclear ribosomal DNA and partial β-tubulin gene sequences. Thirteen morphological and phylogenetic species were identified among the isolates. Leptographium fruticetum, Leptographium abietinum, Ophiostoma bicolor, Ophiostoma manitobense, O. piceaperdum, and eight undescribed species of the genus Ophiostoma and the anamorph genera Leptographium, Hyalorhinocladiella, Ambrosiella and Graphium. A number of these species, i.e. L. fruticetum, Hyalorhinocladiella sp. 2, O. bicolor and O. manitobense, were isolated repeatedly from I. perturbatus, while others, i.e. Graphium sp. 1 and O. piceaperdum, seemed to be sporadic associates. Among all the isolates, L. fruticetum had the highest relative dominance in this survey. A high frequency of occurrence of this species with the beetle may indicate a specific relationship between the two partners.
AB - A number of ophiostomatoid fungi were isolated from the spruce-infesting bark beetle, Ips perturbatus and its galleries collected from felled spruce trees and logs in northern BC and the Yukon Territory. Isolates were identified to species using morphological characteristics, nuclear ribosomal DNA and partial β-tubulin gene sequences. Thirteen morphological and phylogenetic species were identified among the isolates. Leptographium fruticetum, Leptographium abietinum, Ophiostoma bicolor, Ophiostoma manitobense, O. piceaperdum, and eight undescribed species of the genus Ophiostoma and the anamorph genera Leptographium, Hyalorhinocladiella, Ambrosiella and Graphium. A number of these species, i.e. L. fruticetum, Hyalorhinocladiella sp. 2, O. bicolor and O. manitobense, were isolated repeatedly from I. perturbatus, while others, i.e. Graphium sp. 1 and O. piceaperdum, seemed to be sporadic associates. Among all the isolates, L. fruticetum had the highest relative dominance in this survey. A high frequency of occurrence of this species with the beetle may indicate a specific relationship between the two partners.
KW - Ips perturbatus
KW - Ophiostomatoid fungi
KW - Spruce
KW - rDNA
KW - β-tubulin
UR - http://www.scopus.com/inward/record.url?scp=33845264135&partnerID=8YFLogxK
U2 - 10.1007/s10482-006-9092-8
DO - 10.1007/s10482-006-9092-8
M3 - Article
C2 - 17066342
AN - SCOPUS:33845264135
SN - 0003-6072
VL - 91
SP - 19
EP - 34
JO - Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology
JF - Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology
IS - 1
ER -