Opine-based agrobacterium competitiveness

Dual expression control of the agrocinopine catabolism (acc) operon by agrocinopines and phosphate levels

Heenam Kim, Hyojeong Yi, Jaehee Myung, Kevin R. Piper, Stephen K. Farrand

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Agrobacterium tumefaciens strain C58 can transform plant cells to produce and secrete the sugar-phosphate conjugate opines agrocinopines A and B. The bacterium then moves in response to the opines and utilizes them as exclusive sources of carbon, energy, and phosphate via the functions encoded by the acc operon. These privileged opine-involved activities contribute to the formation of agrobacterial niches in the environment. We found that the expression of the acc operon is induced by agrocinopines and also by limitation of phosphate. The main promoter is present in front of the first gene, accR, which codes for a repressor. This operon structure enables efficient repression when opine levels are low. The promoter contains two putative operators, one overlapping the -10 sequence and the other in the further upstream from it; two partly overlapped putative pho boxes between the two operators; and two consecutive transcription start sites. DNA fragments containing either of the operators bound purified repressor AccR in the absence of agrocinopines but not in the presence of the opines, demonstrating the on-off switch of the promoter. Induction of the acc operon can occur under low-phosphate conditions in the absence of agrocinopines and further increases when the opines also are present. Such opine-phosphate dual regulatory system of the operon may ensure maximum utilization of agrocinopines when available and thereby increase the chances of agrobacterial survival in the highly competitive environment with limited general food sources.

Original languageEnglish
Pages (from-to)3700-3711
Number of pages12
JournalJournal of Bacteriology
Volume190
Issue number10
DOIs
Publication statusPublished - 2008 May 1

Fingerprint

Agrobacterium
Operon
Phosphates
Sugar Phosphates
Agrobacterium tumefaciens
agrocinopine
Transcription Initiation Site
Plant Cells
Carbon
Bacteria
Food
DNA

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

Cite this

Opine-based agrobacterium competitiveness : Dual expression control of the agrocinopine catabolism (acc) operon by agrocinopines and phosphate levels. / Kim, Heenam; Yi, Hyojeong; Myung, Jaehee; Piper, Kevin R.; Farrand, Stephen K.

In: Journal of Bacteriology, Vol. 190, No. 10, 01.05.2008, p. 3700-3711.

Research output: Contribution to journalArticle

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