Opine catabolic loci from Agrobacterium plasmids confer chemotaxis to their cognate substrates

Heenam Kim, Stephen K. Farrand

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Opines are carbon compounds produced by crown galls and hairy roots induced by Agrobacterium tumefaciens and A. rhizogenes, respectively. These novel condensation products of plant metabolic intermediates are utilized as nutritional sources by the Agrobacterium strains that induced the growths. Thus, opines are thought to favor the propagation of agrobacteria in the tumorsphere. Certain Agrobacterium strains were chemoattracted to opines. The chemotactic activities to octopine, to nopaline, to mannopine, and to agrocinopines A+B were dependent on the type of the Ti plasmid present in the bacterium. The determinants for chemotaxis to these opines were localized to the regions of the octopine- and nopaline-type Ti plasmids coding for transport and catabolism of that opine. An insertion in accA, which encodes the periplasmic binding protein for agrocinopines A+B, abolished chemotaxis while an insertion in accC, which encodes a component of the transport system, and an insertion in accF, which encodes a function required for agrocinopine catabolism, did not affect chemotaxis to this opine. Thus, transport and catabolism of these opines are not required for the chemotactic activity. Analyses of subclones of the acc region confirmed that accA is the only gene required from the Ti plasmid for chemotaxis to agrocinopines A+B.

Original languageEnglish
Pages (from-to)131-143
Number of pages13
JournalMolecular Plant-Microbe Interactions
Volume11
Issue number2
Publication statusPublished - 1998 Feb 1
Externally publishedYes

Fingerprint

opines
Agrobacterium
Plant Tumor-Inducing Plasmids
chemotaxis
Chemotaxis
plasmids
Plasmids
loci
Substrates
Crown Compounds
Acca
octopine
nopaline
Periplasmic Binding Proteins
Plant Tumors
Agrobacterium tumefaciens
metabolism
Condensation
Bacteria
Carbon

Keywords

  • ABC-type transport system
  • Moc
  • Noc
  • Occ

ASJC Scopus subject areas

  • Microbiology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biotechnology

Cite this

Opine catabolic loci from Agrobacterium plasmids confer chemotaxis to their cognate substrates. / Kim, Heenam; Farrand, Stephen K.

In: Molecular Plant-Microbe Interactions, Vol. 11, No. 2, 01.02.1998, p. 131-143.

Research output: Contribution to journalArticle

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