Opposing Effects on NaV1.2 Function Underlie Differences Between SCN2A Variants Observed in Individuals With Autism Spectrum Disorder or Infantile Seizures

Roy Ben-Shalom, Caroline M. Keeshen, Kiara N. Berrios, Joon-Yong An, Stephan J. Sanders, Kevin J. Bender

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

Background Variants in the SCN2A gene that disrupt the encoded neuronal sodium channel NaV1.2 are important risk factors for autism spectrum disorder (ASD), developmental delay, and infantile seizures. Variants observed in infantile seizures are predominantly missense, leading to a gain of function and increased neuronal excitability. How variants associated with ASD affect NaV1.2 function and neuronal excitability are unclear. Methods We examined the properties of 11 ASD-associated SCN2A variants in heterologous expression systems using whole-cell voltage-clamp electrophysiology and immunohistochemistry. Resultant data were incorporated into computational models of developing and mature cortical pyramidal cells that express NaV1.2. Results In contrast to gain of function variants that contribute to seizure, we found that all ASD-associated variants dampened or eliminated channel function. Incorporating these electrophysiological results into a compartmental model of developing excitatory neurons demonstrated that all ASD variants, regardless of their mechanism of action, resulted in deficits in neuronal excitability. Corresponding analysis of mature neurons predicted minimal change in neuronal excitability. Conclusions This functional characterization thus identifies SCN2A mutation and NaV1.2 dysfunction as the most frequently observed ASD risk factor detectable by exome sequencing and suggests that associated changes in neuronal excitability, particularly in developing neurons, may contribute to ASD etiology.

Original languageEnglish
Pages (from-to)224-232
Number of pages9
JournalBiological Psychiatry
Volume82
Issue number3
DOIs
Publication statusPublished - 2017 Aug 1
Externally publishedYes

Fingerprint

Seizures
Neurons
Exome
Sodium Channels
Pyramidal Cells
Electrophysiology
Autism Spectrum Disorder
Immunohistochemistry
Mutation
Genes

Keywords

  • Autism spectrum disorder
  • electrophysiology
  • Epilepsy
  • Na1.2
  • SCN2A
  • Seizure

ASJC Scopus subject areas

  • Biological Psychiatry

Cite this

Opposing Effects on NaV1.2 Function Underlie Differences Between SCN2A Variants Observed in Individuals With Autism Spectrum Disorder or Infantile Seizures. / Ben-Shalom, Roy; Keeshen, Caroline M.; Berrios, Kiara N.; An, Joon-Yong; Sanders, Stephan J.; Bender, Kevin J.

In: Biological Psychiatry, Vol. 82, No. 3, 01.08.2017, p. 224-232.

Research output: Contribution to journalArticle

Ben-Shalom, Roy ; Keeshen, Caroline M. ; Berrios, Kiara N. ; An, Joon-Yong ; Sanders, Stephan J. ; Bender, Kevin J. / Opposing Effects on NaV1.2 Function Underlie Differences Between SCN2A Variants Observed in Individuals With Autism Spectrum Disorder or Infantile Seizures. In: Biological Psychiatry. 2017 ; Vol. 82, No. 3. pp. 224-232.
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