Optical diffraction tomography for high resolution live cell imaging

Yongjin Sung, Wonshik Choi, Christopher Fang-Yen, Kamran Badizadegan, Ramachandra R. Dasari, Michael S. Feld

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We report the experimental implementation of optical diffraction tomography for quantitative 3D mapping of refractive index in live biological cells. To quantitatively reconstruct the 3D map of complex refractive index in live cells, we apply optical diffraction tomography based on the Rytov approximation. In this way, the effect of diffraction is taken into account in the reconstruction process and diffraction-free high resolution 3D images are obtained throughout the entire sample volume. The quantitative refractive index map can potentially serve as an intrinsic assay to provide the molecular concentrations without the addition of exogenous agents and also to provide a method for studying the light scattering properties of single cells.

Original languageEnglish
Title of host publicationOptics InfoBase Conference Papers
Publication statusPublished - 2009 Dec 1
Externally publishedYes
EventNovel Techniques in Microscopy, NTM 2009 - Vancouver, Canada
Duration: 2009 Apr 262009 Apr 30

Other

OtherNovel Techniques in Microscopy, NTM 2009
CountryCanada
CityVancouver
Period09/4/2609/4/30

Fingerprint

Tomography
tomography
Diffraction
Imaging techniques
Refractive index
high resolution
refractivity
cells
diffraction
Light scattering
Assays
light scattering
approximation

ASJC Scopus subject areas

  • Instrumentation
  • Atomic and Molecular Physics, and Optics

Cite this

Sung, Y., Choi, W., Fang-Yen, C., Badizadegan, K., Dasari, R. R., & Feld, M. S. (2009). Optical diffraction tomography for high resolution live cell imaging. In Optics InfoBase Conference Papers

Optical diffraction tomography for high resolution live cell imaging. / Sung, Yongjin; Choi, Wonshik; Fang-Yen, Christopher; Badizadegan, Kamran; Dasari, Ramachandra R.; Feld, Michael S.

Optics InfoBase Conference Papers. 2009.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Sung, Y, Choi, W, Fang-Yen, C, Badizadegan, K, Dasari, RR & Feld, MS 2009, Optical diffraction tomography for high resolution live cell imaging. in Optics InfoBase Conference Papers. Novel Techniques in Microscopy, NTM 2009, Vancouver, Canada, 09/4/26.
Sung Y, Choi W, Fang-Yen C, Badizadegan K, Dasari RR, Feld MS. Optical diffraction tomography for high resolution live cell imaging. In Optics InfoBase Conference Papers. 2009
Sung, Yongjin ; Choi, Wonshik ; Fang-Yen, Christopher ; Badizadegan, Kamran ; Dasari, Ramachandra R. ; Feld, Michael S. / Optical diffraction tomography for high resolution live cell imaging. Optics InfoBase Conference Papers. 2009.
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