TY - JOUR
T1 - Optical diffraction tomography for high resolution live cell imaging
AU - Sung, Yongjin
AU - Choi, Wonshik
AU - Fang-Yen, Christopher
AU - Badizadegan, Kamran
AU - Dasari, Ramachandra R.
AU - Feld, Michael S.
N1 - Copyright:
Copyright 2018 Elsevier B.V., All rights reserved.
PY - 2009/1/5
Y1 - 2009/1/5
N2 - We report the experimental implementation of optical diffraction tomography for quantitative 3D mapping of refractive index in live biological cells. Using a heterodyne Mach-Zehnder interferometer, we record complex field images of light transmitted through a sample with varying directions of illumination. To quantitatively reconstruct the 3D map of complex refractive index in live cells, we apply optical diffraction tomography based on the Rytov approximation. In this way, the effect of diffraction is taken into account in the reconstruction process and diffraction-free high resolution 3D images are obtained throughout the entire sample volume. The quantitative refractive index map can potentially serve as an intrinsic assay to provide the molecular concentrations without the addition of exogenous agents and also to provide a method for studying the light scattering properties of single cells.
AB - We report the experimental implementation of optical diffraction tomography for quantitative 3D mapping of refractive index in live biological cells. Using a heterodyne Mach-Zehnder interferometer, we record complex field images of light transmitted through a sample with varying directions of illumination. To quantitatively reconstruct the 3D map of complex refractive index in live cells, we apply optical diffraction tomography based on the Rytov approximation. In this way, the effect of diffraction is taken into account in the reconstruction process and diffraction-free high resolution 3D images are obtained throughout the entire sample volume. The quantitative refractive index map can potentially serve as an intrinsic assay to provide the molecular concentrations without the addition of exogenous agents and also to provide a method for studying the light scattering properties of single cells.
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U2 - 10.1364/OE.17.000266
DO - 10.1364/OE.17.000266
M3 - Article
C2 - 19129896
AN - SCOPUS:58449127269
VL - 17
SP - 266
EP - 277
JO - Optics Express
JF - Optics Express
SN - 1094-4087
IS - 1
ER -