Optical imaging featuring both long working distance and high spatial resolution by correcting the aberration of a large aperture lens

Changsoon Choi, Kyung Deok Song, Sungsam Kang, Jin Sung Park, Wonshik Choi

Research output: Contribution to journalArticle

Abstract

High-resolution optical imaging within thick objects has been a challenging task due to the short working distance of conventional high numerical aperture (NA) objective lenses. Lenses with a large physical diameter and thus a large aperture, such as microscope condenser lenses, can feature both a large NA and a long working distance. However, such lenses suffer from strong aberrations. To overcome this problem, we present a method to correct the aberrations of a transmission-mode imaging system that is composed of two condensers. The proposed method separately identifies and corrects aberrations of illumination and collection lenses of up to 1.2 NA by iteratively optimizing the total intensity of the synthetic aperture images in the forward and phase-conjugation processes. At a source wavelength of 785 nm, we demonstrated a spatial resolution of 372 nm at extremely long working distances of up to 1.6 mm, an order of magnitude improvement in comparison to conventional objective lenses. Our method of converting microscope condensers to high-quality objectives may facilitate increases in the imaging depths of super-resolution and expansion microscopes.

Original languageEnglish
Article number9165
JournalScientific Reports
Volume8
Issue number1
DOIs
Publication statusPublished - 2018 Dec 1

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aberration
spatial resolution
apertures
lenses
high resolution
condensers
numerical aperture
microscopes
synthetic apertures
phase conjugation
illumination
expansion
wavelengths

ASJC Scopus subject areas

  • General

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Optical imaging featuring both long working distance and high spatial resolution by correcting the aberration of a large aperture lens. / Choi, Changsoon; Song, Kyung Deok; Kang, Sungsam; Park, Jin Sung; Choi, Wonshik.

In: Scientific Reports, Vol. 8, No. 1, 9165, 01.12.2018.

Research output: Contribution to journalArticle

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