Pseudomonas sp. PA09 was isolated from farm soil and used for the optical resolution of D-pipecolic acid from DL-pipecolic acid. The strain PA09 consumed L-pipecolic acid preferentially as the sole carbon and energy source, thus accumulating D-pipecolic acid in the culture broth. Optimization to improve the enantiomeric excess and yield was performed. The time course experiment showed that the strain PA09 consumed L-pipecolic acid almost to completion after 25 h of cultivation, and the enantiomeric excess and the yield (% of residual D-pipecolic acid) were 99.8 and 96.0%, respectively.
|Number of pages||5|
|Journal||Journal of microbiology and biotechnology|
|Publication status||Published - 2001 Apr|
- D-pipecolic acid
- Optical resolution
- Pseudomonas sp.
ASJC Scopus subject areas
- Applied Microbiology and Biotechnology