Optical resolution of DL-pipecolic acid by fermentation using Pseudomonas sp. PA09

Chan Soo Kim, Il Seok Lee, Namhyun Chung, Won Gi Bang

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Pseudomonas sp. PA09 was isolated from farm soil and used for the optical resolution of D-pipecolic acid from DL-pipecolic acid. The strain PA09 consumed L-pipecolic acid preferentially as the sole carbon and energy source, thus accumulating D-pipecolic acid in the culture broth. Optimization to improve the enantiomeric excess and yield was performed. The time course experiment showed that the strain PA09 consumed L-pipecolic acid almost to completion after 25 h of cultivation, and the enantiomeric excess and the yield (% of residual D-pipecolic acid) were 99.8 and 96.0%, respectively.

Original languageEnglish
Pages (from-to)217-221
Number of pages5
JournalJournal of Microbiology and Biotechnology
Volume11
Issue number2
Publication statusPublished - 2001 Apr 1

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Keywords

  • D-pipecolic acid
  • Fermentation
  • Optical resolution
  • Pseudomonas sp.

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology
  • Microbiology

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