Optimal conditions for the production of immunostimulating polysaccharides from the suspension culture of Angelica gigas cells

Kyung Seop Ahn, Weon Taek Seo, Woong Seop Sim, Ik Hwan Kim

Research output: Contribution to journalArticle

Abstract

An Immunostimulating polysaccharide was produced from the suspension culture of Angelica gigas H4, plant cells. In order to enhance the polysaccharide production by the A. gigas cell culture, medium composition and physical conditions were optimized. Schenk and Hildebrandt (SH) medium was selected as an optimal basal medium for the growth of A. gigas. The maximum cell and polysaccharide concentration obtained in SH medium were 15.8 g DCW/l and 0.85g polysaccharide/l, respectively, at 25°C under dark condition. For the enhanced polysaccharide production, a polysaccharide production medium (PPM) was established by modifying Gamborg B5 medium with optimized carbon sources, growth regulators, organic and inorganic elements. Optimal initial pH and temperature were 6.0-6.6 and 20°C, respectively, and the dark condition was better than the light condition. The maximum polysaccharide concentration of 1.5 g/l could be obtained through the optimization of the medium composition and physical conditions.

Original languageEnglish
Pages (from-to)130-136
Number of pages7
JournalKorean Journal of Applied Microbiology and Biotechnology
Volume26
Issue number2
Publication statusPublished - 1998 Apr 1

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Angelica
Polysaccharides
Suspensions
Plant Cells
Growth
Culture Media
Carbon
Cell Culture Techniques
Light
Temperature

Keywords

  • Angelica gigas Nakai
  • Immunostimulating polysaccharides
  • Polysaccharide production medium (PPM)
  • Suspension culture

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

Cite this

Optimal conditions for the production of immunostimulating polysaccharides from the suspension culture of Angelica gigas cells. / Ahn, Kyung Seop; Seo, Weon Taek; Sim, Woong Seop; Kim, Ik Hwan.

In: Korean Journal of Applied Microbiology and Biotechnology, Vol. 26, No. 2, 01.04.1998, p. 130-136.

Research output: Contribution to journalArticle

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AB - An Immunostimulating polysaccharide was produced from the suspension culture of Angelica gigas H4, plant cells. In order to enhance the polysaccharide production by the A. gigas cell culture, medium composition and physical conditions were optimized. Schenk and Hildebrandt (SH) medium was selected as an optimal basal medium for the growth of A. gigas. The maximum cell and polysaccharide concentration obtained in SH medium were 15.8 g DCW/l and 0.85g polysaccharide/l, respectively, at 25°C under dark condition. For the enhanced polysaccharide production, a polysaccharide production medium (PPM) was established by modifying Gamborg B5 medium with optimized carbon sources, growth regulators, organic and inorganic elements. Optimal initial pH and temperature were 6.0-6.6 and 20°C, respectively, and the dark condition was better than the light condition. The maximum polysaccharide concentration of 1.5 g/l could be obtained through the optimization of the medium composition and physical conditions.

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