Optimal Conditions to Remove Chemical Hazards in Fish Protein Isolates from Tilapia Frame Using Response Surface Methodology

Hathaigan Kokkaew, Supawan Thawornchinsombut, Jae W. Park, Theparit Pitirit

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Response surface methodology (RSM) was employed to maximize the removal of phospholipids (PLs) and other chemical hazards in tilapia protein isolates made from tilapia frame (TF). CaCl2 and the ratio of water to minced tilapia frame (W:TF) were the significant variables affecting PLs reduction. The optimum condition for maximal PLs reduction (90.0%) was: 10.25 mM CaCl2 and a W:TF of 7.8:1, while other variables were fixed at 5 mM citric acid, 60 min incubation, pH 11, and centrifugal speed of 8,000 × g. At these conditions, the great reduction of lipids (93.9%), Hg (97.6%), and As (95.5%), as well as 86.1% of protein recovery, were obtained. Protein isolates with significantly reduced chemical hazards and lipids were successfully prepared from tilapia frames using the alkaline extraction assisted with CaCl2 and citric acid.

Original languageEnglish
JournalJournal of Aquatic Food Product Technology
DOIs
Publication statusAccepted/In press - 2015 Aug 27
Externally publishedYes

Keywords

  • fish protein isolate
  • hazardous contaminants
  • phospholipids
  • response surface methodology
  • tilapia by-product

ASJC Scopus subject areas

  • Food Science
  • Aquatic Science

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