Optimal production of 4-deoxy-L-erythro-5-hexoseulose uronic acid from alginate for brown macro algae saccharification by combining endo- and exo-type alginate lyases

Da M ao Wang, Hee T aek Kim, Eun J u Yun, Do H young Kim, Yong Cheol Park, Hee C hul Woo, Kyoung Heon Kim

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Algae are considered as third-generation biomass, and alginate is the main component of brown macroalgae. Alginate can be enzymatically depolymerized by alginate lyases into uronate monomers, such as mannuronic acid and guluronic acid, which are further nonenzymatically converted to 4-deoxy-L-erythro-5-hexoseulose uronic acid (DEH). We have optimized an enzymatic saccharification process using two recombinant alginate lyases, endo-type Alg7D and exo-type Alg17C, for the efficient production of DEH from alginate. When comparing the sequential and simultaneous additions of Alg7D and Alg17C, it was found that the final yield of DEH was significantly higher when the enzymes were added sequentially. The progress of saccharification reactions and production of DEH were verified by thin layer chromatography and gas chromatography-mass spectrometry, respectively. Our results showed that the two recombinant enzymes could be exploited for the efficient production of DEH that is the key substrate for producing biofuels from brown macro algal biomass.

Original languageEnglish
Pages (from-to)2105-2111
Number of pages7
JournalBioprocess and Biosystems Engineering
Volume37
Issue number10
DOIs
Publication statusPublished - 2014 Oct 1

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Phaeophyta
Uronic Acids
Saccharification
Alginate
Algae
Macros
Biomass
Acids
Seaweed
Thin layer chromatography
Biofuels
Enzymes
Thin Layer Chromatography
Gas chromatography
Gas Chromatography-Mass Spectrometry
Mass spectrometry
Monomers
Substrates
alginic acid
poly(beta-D-mannuronate) lyase

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Optimal production of 4-deoxy-L-erythro-5-hexoseulose uronic acid from alginate for brown macro algae saccharification by combining endo- and exo-type alginate lyases. / Wang, Da M ao; Kim, Hee T aek; Yun, Eun J u; Kim, Do H young; Park, Yong Cheol; Woo, Hee C hul; Kim, Kyoung Heon.

In: Bioprocess and Biosystems Engineering, Vol. 37, No. 10, 01.10.2014, p. 2105-2111.

Research output: Contribution to journalArticle

Wang, Da M ao ; Kim, Hee T aek ; Yun, Eun J u ; Kim, Do H young ; Park, Yong Cheol ; Woo, Hee C hul ; Kim, Kyoung Heon. / Optimal production of 4-deoxy-L-erythro-5-hexoseulose uronic acid from alginate for brown macro algae saccharification by combining endo- and exo-type alginate lyases. In: Bioprocess and Biosystems Engineering. 2014 ; Vol. 37, No. 10. pp. 2105-2111.
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