Optimal production of 7,10-dihydroxy-8(E)-hexadecenoic acid from palmitoleic acid by Pseudomonas aeruginosa PR3

Jae H. Bae, Min Jung Suh, Beom S. Kim, Ching T. Hou, In Jung Lee, In-Hwan Kim, Hak Ryul Kim

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The hydroxylation of unsaturated fatty acids by bacterial strains is one type of value-adding bioconversion processes. This process generates new hydroxy fatty acids (HFA) carrying special properties such as higher viscosity and reactivity compared with normal fatty acids. Among microbial strains tested for HFA production, Pseudomonas aeruginosa PR3 is well known to utilize various unsaturated fatty acids to produce mono-, di- and tri-hydroxy fatty acids. Previously we reported that strain PR3 could produce a novel value-added hydroxy fatty acid 7,10-dihydroxy-8(E)-hexadecenoic acid (DHD) from palmitoleic acid (Bae et al. (2007) Appl. Microbiol. Biotechnol. 75, 435-440). In this study, we focused on the development of the optimal nutritional and environmental conditions for DHD production from palmitoleic acid by PR3. Optimal carbon and nitrogen sources for DHD production were fructose and yeast extract, respectively. Optimal initial medium pH and incubation temperature were pH 8.0 and 30°C and magnesium ion was essentially required for DHD production. Substrate concentration and time of substrate addition were also optimized. Under optimized conditions, maximal DHD production was 1600. mg/l representing 26.7% conversion yield.

Original languageEnglish
Pages (from-to)352-357
Number of pages6
JournalNew Biotechnology
Volume27
Issue number4
DOIs
Publication statusPublished - 2010 Sep 1

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Hydroxy Acids
Fatty acids
Pseudomonas aeruginosa
Fatty Acids
Acids
Unsaturated fatty acids
Unsaturated Fatty Acids
Bioconversion
Hydroxylation
Fructose
Substrates
Viscosity
Yeast
Magnesium
Nitrogen
Carbon
Yeasts
palmitoleic acid
7,10-dihydroxy-8-hexadecenoic acid
hexadecenoic acid

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Biology
  • Bioengineering

Cite this

Optimal production of 7,10-dihydroxy-8(E)-hexadecenoic acid from palmitoleic acid by Pseudomonas aeruginosa PR3. / Bae, Jae H.; Suh, Min Jung; Kim, Beom S.; Hou, Ching T.; Lee, In Jung; Kim, In-Hwan; Kim, Hak Ryul.

In: New Biotechnology, Vol. 27, No. 4, 01.09.2010, p. 352-357.

Research output: Contribution to journalArticle

Bae, Jae H. ; Suh, Min Jung ; Kim, Beom S. ; Hou, Ching T. ; Lee, In Jung ; Kim, In-Hwan ; Kim, Hak Ryul. / Optimal production of 7,10-dihydroxy-8(E)-hexadecenoic acid from palmitoleic acid by Pseudomonas aeruginosa PR3. In: New Biotechnology. 2010 ; Vol. 27, No. 4. pp. 352-357.
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