Optimal strategy of pH control in the production of recombinant human epidermal growth factor by Hansenula polymorpha

H. Moon; H. Kim; S. K. Rhee; E. S. Choi; I. H. Kim; S. I. Hong

doi:10.1016/S0032-9592(02)00172-3

Optimal strategy of pH control in the production of recombinant human epidermal growth factor by Hansenula polymorpha

H. Moon, H. Kim, S. K. Rhee, E. S. Choi, I. H. Kim, S. I. Hong

Department of Biotechnology

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

The pH dependency of cell growth and human epidermal growth factor (hEGF) production was studied with a recombinant Hansenula polymorpha strain. Maximum specific growth rate and apparent specific production rate of hEGF was highly dependent on culture pH over the range of 4,0-7.0. The optimal pH for the fastest growth was 5.0 and for the apparent production of hEGF 6.0. A simple kinetic model considering the degradation of hEGF during culture was established. Then it was used to estimate the intrinsic specific production rate of hEGF and simulate expression behavior according to culture pH. The intrinsic specific production rate of hEGF was relatively unaffected by culture pH over the range of 5.0-7.0. An optimal pH switching time exists for a given methanol concentration in the fed-batch culture maintaining constant methanol concentration.

Original language	English
Pages (from-to)	487-495
Number of pages	9
Journal	Process Biochemistry
Volume	38
Issue number	4
DOIs	https://doi.org/10.1016/S0032-9592(02)00172-3
Publication status	Published - 2002 Dec 2

Keywords

Culture pH
Degradation
Hansenula polymorpha
Human epidermal growth factor
Modeling
pH control

ASJC Scopus subject areas

Bioengineering
Biochemistry
Applied Microbiology and Biotechnology

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10.1016/S0032-9592(02)00172-3

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@article{72aa11a3cc044d32bbcb37bd4adeaf72,

title = "Optimal strategy of pH control in the production of recombinant human epidermal growth factor by Hansenula polymorpha",

abstract = "The pH dependency of cell growth and human epidermal growth factor (hEGF) production was studied with a recombinant Hansenula polymorpha strain. Maximum specific growth rate and apparent specific production rate of hEGF was highly dependent on culture pH over the range of 4,0-7.0. The optimal pH for the fastest growth was 5.0 and for the apparent production of hEGF 6.0. A simple kinetic model considering the degradation of hEGF during culture was established. Then it was used to estimate the intrinsic specific production rate of hEGF and simulate expression behavior according to culture pH. The intrinsic specific production rate of hEGF was relatively unaffected by culture pH over the range of 5.0-7.0. An optimal pH switching time exists for a given methanol concentration in the fed-batch culture maintaining constant methanol concentration.",

keywords = "Culture pH, Degradation, Hansenula polymorpha, Human epidermal growth factor, Modeling, pH control",

author = "H. Moon and H. Kim and Rhee, {S. K.} and Choi, {E. S.} and Kim, {I. H.} and Hong, {S. I.}",

note = "Funding Information: This work was supported by research grants from the KOSEF through Applied Rheology Center at Korea University, Korea. ",

year = "2002",

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doi = "10.1016/S0032-9592(02)00172-3",

language = "English",

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journal = "Process Biochemistry",

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T1 - Optimal strategy of pH control in the production of recombinant human epidermal growth factor by Hansenula polymorpha

AU - Moon, H.

AU - Kim, H.

AU - Rhee, S. K.

AU - Choi, E. S.

AU - Kim, I. H.

AU - Hong, S. I.

N1 - Funding Information: This work was supported by research grants from the KOSEF through Applied Rheology Center at Korea University, Korea.

PY - 2002/12/2

Y1 - 2002/12/2

N2 - The pH dependency of cell growth and human epidermal growth factor (hEGF) production was studied with a recombinant Hansenula polymorpha strain. Maximum specific growth rate and apparent specific production rate of hEGF was highly dependent on culture pH over the range of 4,0-7.0. The optimal pH for the fastest growth was 5.0 and for the apparent production of hEGF 6.0. A simple kinetic model considering the degradation of hEGF during culture was established. Then it was used to estimate the intrinsic specific production rate of hEGF and simulate expression behavior according to culture pH. The intrinsic specific production rate of hEGF was relatively unaffected by culture pH over the range of 5.0-7.0. An optimal pH switching time exists for a given methanol concentration in the fed-batch culture maintaining constant methanol concentration.

AB - The pH dependency of cell growth and human epidermal growth factor (hEGF) production was studied with a recombinant Hansenula polymorpha strain. Maximum specific growth rate and apparent specific production rate of hEGF was highly dependent on culture pH over the range of 4,0-7.0. The optimal pH for the fastest growth was 5.0 and for the apparent production of hEGF 6.0. A simple kinetic model considering the degradation of hEGF during culture was established. Then it was used to estimate the intrinsic specific production rate of hEGF and simulate expression behavior according to culture pH. The intrinsic specific production rate of hEGF was relatively unaffected by culture pH over the range of 5.0-7.0. An optimal pH switching time exists for a given methanol concentration in the fed-batch culture maintaining constant methanol concentration.

KW - Culture pH

KW - Degradation

KW - Hansenula polymorpha

KW - Human epidermal growth factor

KW - Modeling

KW - pH control

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U2 - 10.1016/S0032-9592(02)00172-3

DO - 10.1016/S0032-9592(02)00172-3

M3 - Article

AN - SCOPUS:0037010798

SN - 1359-5113

VL - 38

SP - 487

EP - 495

JO - Process Biochemistry

JF - Process Biochemistry

IS - 4

ER -

Optimal strategy of pH control in the production of recombinant human epidermal growth factor by Hansenula polymorpha

Abstract

Keywords

ASJC Scopus subject areas

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