Optimization and validation of a fluorogenic dipeptidyl peptidase 4 enzymatic assay in human plasma

Medicine & Life Sciences

• Dipeptidyl Peptidase 4 100%
• Enzyme Assays 84%
• 4-(3-amino-4-(2,4,5-trifluorophenyl)butanoyl)-3-(tert-butoxymethyl)piperazin-2-one 38%
• Calibration 19%
• glycylproline 16%
• tartaric acid 13%
• Dipeptidyl-Peptidase IV Inhibitors 12%
• Metformin 9%
• Freezing 9%
• Type 2 Diabetes Mellitus 6%
• Kinetics 6%
• Temperature 6%

Chemical Compounds

• Plasma 45%
• Velocity 37%
• Enzyme Activity 36%
• Analytical Calibration 25%
• Dipeptidyl Peptidase IV Inhibitor 23%
• Metformin 21%
• Tartrate 17%
• Enzymatic Reaction 14%
• Dilution 11%
• Time 5%