Optimization of β-glucosidase production by a strain of Stereum hirsutum and its application in enzymatic saccharification.

Priyadharshini Ramachandran, Ngoc Phuong Thao Nguyen, Joon Ho Choi, Yun Chan Kang, Marimuthu Jeya, Jung Kul Lee

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

A high beta-glucosidase (BGL)-producing strain, Stereum hirsutum, was identified and isolated and showed a maximum BGL activity (10.4 U/ml) when cultured with Avicel and tryptone as the carbon and nitrogen sources, respectively. In comparison with other BGLs, BGL obtained from S. hirsutum showed a higher level of activity to cellobiose (V(max) = 172 U/mg, and k(cat) = 281/s). Under the optimum conditions (600 rpm, 30°C, and pH 6.0), the maximum BGL activity of 10.4 U/ml with the overall productivity of 74.5 U/l/h was observed. BGL production was scaled up from a laboratory scale (7-L fermenter) to a pilot scale (70-L fermenter). When S. hirsutum was cultured in fed-batch culture with rice straw as the carbon source in a 70-L fermenter, a comparable productivity of 78.6 U/l/h was obtained. Furthermore, S. hirsutum showed high levels of activity of other lignocellulases (cellobiohydrolase, endoglucanase, xylanase, and laccase) that are involved in the saccharification of biomasses. Application of S. hirsutum lignocellulases in the hydrolysis of Pinus densiflora and Catalpa ovata showed saccharification yields of 49.7% and 43.0%, respectively, which were higher than the yield obtained using commercial enzymes.

Original languageEnglish
Pages (from-to)351-356
Number of pages6
JournalUnknown Journal
Volume23
Issue number3
DOIs
Publication statusPublished - 2013 Jan 1

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Glucosidases
Saccharification
beta-Glucosidase
Fermenters
Carbon
Productivity
Cellulose 1,4-beta-Cellobiosidase
Cellobiose
Laccase
Pinus
Batch Cell Culture Techniques
Cellulase
Straw
Cellulose
Biomass
Hydrolysis
Nitrogen
Enzymes

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

Cite this

Optimization of β-glucosidase production by a strain of Stereum hirsutum and its application in enzymatic saccharification. / Ramachandran, Priyadharshini; Nguyen, Ngoc Phuong Thao; Choi, Joon Ho; Kang, Yun Chan; Jeya, Marimuthu; Lee, Jung Kul.

In: Unknown Journal, Vol. 23, No. 3, 01.01.2013, p. 351-356.

Research output: Contribution to journalArticle

Ramachandran, Priyadharshini ; Nguyen, Ngoc Phuong Thao ; Choi, Joon Ho ; Kang, Yun Chan ; Jeya, Marimuthu ; Lee, Jung Kul. / Optimization of β-glucosidase production by a strain of Stereum hirsutum and its application in enzymatic saccharification. In: Unknown Journal. 2013 ; Vol. 23, No. 3. pp. 351-356.
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