Optimization of butanol production of Clostridium pasteurianum using response. Surface methodology

C. Moon, B. I. Sang, Y. Um, S. W. Kim

Research output: Contribution to conferencePaper

Abstract

The optimization of fermentation conditions for converting glycerol to butanol was studied with Clostridium pasteurianum DSM525, which is known to convert glycerol to butanol and 1,3-propanediol (1,3-PD). Based on one-factor-at-a-time studies, pH, glycerol concentration, and the addition of co-substrate such as butyrate significantly influenced cell growth and butanol production by C. pasteurianum DSM525 in batch cultures. When 2-(N-Morpholino)ethanesulfonic acid (MES) was added to reduce pH drop during batch fermentations, butanol production was higher than 1,3-PD production without MES addition. However, butanol concentration was lower than 1,3-PD. The addition of co-substrate, such as butyrate, enhanced the production of butanol by > 50%. The concentration of carbon source, glycerol, also significantly affected cell growth and butanol production. The concentration of glycerol and butyrate, and ph were the three important variables to impact butanol production of C. pasteurianum DSM525. Further screening experiments will be performed to identify other important variables that significantly influence butanol production of C. pasteurianum DSM525. This is an abstract of a paper presented at the 8th World Congress of Chemical Engineering (Montréal, Quebec, Canada 8/23-27/2009).

Original languageEnglish
Pages520b
Publication statusPublished - 2009
Event8th World Congress of Chemical Engineering: Incorporating the 59th Canadian Chemical Engineering Conference and the 24th Interamerican Congress of Chemical Engineering - Montreal, QC, Canada
Duration: 2009 Aug 232009 Aug 27

Other

Other8th World Congress of Chemical Engineering: Incorporating the 59th Canadian Chemical Engineering Conference and the 24th Interamerican Congress of Chemical Engineering
CountryCanada
CityMontreal, QC
Period09/8/2309/8/27

ASJC Scopus subject areas

  • Biotechnology
  • Chemical Health and Safety
  • Process Chemistry and Technology
  • Renewable Energy, Sustainability and the Environment

Fingerprint Dive into the research topics of 'Optimization of butanol production of Clostridium pasteurianum using response. Surface methodology'. Together they form a unique fingerprint.

  • Cite this

    Moon, C., Sang, B. I., Um, Y., & Kim, S. W. (2009). Optimization of butanol production of Clostridium pasteurianum using response. Surface methodology. 520b. Paper presented at 8th World Congress of Chemical Engineering: Incorporating the 59th Canadian Chemical Engineering Conference and the 24th Interamerican Congress of Chemical Engineering, Montreal, QC, Canada.