Optimization of endoglucanase production by Trichoderma harzianum KUC1716 and enzymatic hydrolysis of lignocellulosic biomass

Hanbyul Lee, Young Min Lee, Young Mok Heo, Hwanhwi Lee, Joo Hyun Hong, Seokyoon Jang, Mihee Min, Jaejung Lee, Jun Seok Kim, Gyu Hyeok Kim, Jae Jin Kim

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)

Abstract

Trichoderma species are widely used for the commercial production of cellulolytic enzymes. In the present investigation, medium components were optimized using a central composite design and response surface methodology to produce endoglucanase (EG) from Trichoderma harzianum KUC1716. From the various medium components tested, cellulose, soy peptone, and thiamine HCI were selected as the optimal carbon, nitrogen, and vitamin sources, respectively. The highest EG (1.97 U/mL) production was obtained with 1.85% cellulose, 0.48% soy peptone, and 0.10% thiamine HCI. EG production in the optimized medium was 2.6 fold higher than in the unoptimized medium. In addition, the crude enzyme preparation from T. harzianum KUC1716 supplemented with (3-glucosidase from Schizophyllum commune KUC9397 was used to hydrolyze various types of lignocellulosic materials and showed significant saccharification yields on all lignocellulosic materials, surpassing that of a commercial enzyme cocktail. It was verified that the crude enzyme preparation derived from T. harzianum KUC1716 could replace the commercial enzymes. This highlights the potential of the crude enzymes for use in biomass conversion systems.

Original languageEnglish
Pages (from-to)7466-7476
Number of pages11
JournalBioResources
Volume10
Issue number4
DOIs
Publication statusPublished - 2015

Keywords

  • Endoglucanase
  • Optimization
  • Response surface methodology
  • Saccharification
  • Trichoderma harzianum

ASJC Scopus subject areas

  • Environmental Engineering
  • Bioengineering
  • Waste Management and Disposal

Fingerprint

Dive into the research topics of 'Optimization of endoglucanase production by Trichoderma harzianum KUC1716 and enzymatic hydrolysis of lignocellulosic biomass'. Together they form a unique fingerprint.

Cite this