Optimization of enzymatic biodiesel synthesis using RSM in high pressure carbon dioxide and its scale up

Myunggu Lee, Dohoon Lee, Jaehoon Cho, Junhac Lee, Sangyong Kim, Seung Wook Kim, Chulhwan Park

Research output: Contribution to journalArticle

4 Citations (Scopus)


Enzymatic synthesis of biodiesel by the transesterification of canola oil and methanol in high pressure carbon dioxide [HPCO2: near-critical and supercritical carbon dioxide (NcCO2 and ScCO2)] was optimized using response surface methodology (RSM). RSM based on 5-level-5-factor central composite rotatable design (CCRD) was used to evaluate the effects of temperature, pressure, enzyme loading, substrate molar ratio, and time on the conversion to biodiesel by transesterification. Finally, batch reactions for biodiesel synthesis were preformed in a 100 mL and 7 L high-pressure stirred batch reactors.

Original languageEnglish
Pages (from-to)775-780
Number of pages6
JournalBioprocess and Biosystems Engineering
Issue number6
Publication statusPublished - 2013 Jun 1



  • High pressure carbon dioxide
  • Lipase
  • Optimization
  • Response surface methodology
  • Transesterification

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering

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