Optimization of hydrogen production by hyperthermophilic eubacteria, Thermotoga maritima and Thermotoga neapolitana in batch fermentation

Tam Anh D. Nguyen, Jun Pyo Kim, Mi Sun Kim, You Kwan Oh, Sang Jun Sim

Research output: Contribution to journalArticle

68 Citations (Scopus)

Abstract

The batch fermentations of two hyperthermophilic eubacteria Thermotoga maritima strain DSM 3109 and Thermotoga neapolitana strain DSM 4359 were carried out to optimize the hydrogen production. The simple and economical culture medium using cheap salts with strong buffering capacity was designed based on T. maritima basal medium (TMB). Both strains cultivated under strictly anaerobic conditions showed the best growth at temperature of 75-80 °C and pH of 6.5-7.0. The maximum cell growth of 3.14 g DCW/L and hydrogen production of 342 mL H2 gas/L were obtained, respectively, in the modified TB medium containing 7.5 g/L of glucose and 4 g/L of yeast extract. Hydrogen accumulation in the headspace was more than 30% of the gaseous phase. Cells were also cultivated in cellulose-containing medium to test the feasibility of hydrogen production.

Original languageEnglish
Pages (from-to)1483-1488
Number of pages6
JournalInternational Journal of Hydrogen Energy
Volume33
Issue number5
DOIs
Publication statusPublished - 2008 Mar
Externally publishedYes

Keywords

  • Hydrogen production
  • Hyperthermophilic eubacteria
  • Thermotoga

ASJC Scopus subject areas

  • Renewable Energy, Sustainability and the Environment
  • Fuel Technology
  • Condensed Matter Physics
  • Energy Engineering and Power Technology

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