Optimization of lipase pretreatment prior to lipase immobilization to prevent loss of activity

Dong Hwan Lee, Jung Mo Kim, Hyun Yong Shin, Seung Wook Kim

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

In our previous work, a method of pretreating lipase was developed to prevent loss of its activity during covalent immobilization. In this study, Rhizopus oryzae lipase was pretreated before immobilization and then immobilized on a silica gel surface. The effects of the various materials and conditions used in the pretreatment stage on the activity of immobilized lipase were investigated. Immobilized lipase pretreated with 0.1% of soybean oil had better activity than those pretreated with other materials. The optimal temperature, agitation speed, and pretreating time for lipase pretreatment were determined to be 40°C, 200 rpm, and 45 min, respectively. The activity of immobilized soybean oil pretreated lipase was 630 U/g matrix, which is 20 times higher than that of immobilized non-pretreated lipase. In addition, immobilized lipase activity was maintained at levels exceeding 90% of its original activity after 10 reuses.

Original languageEnglish
Pages (from-to)650-654
Number of pages5
JournalJournal of Microbiology and Biotechnology
Volume17
Issue number4
Publication statusPublished - 2007 Apr 1

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Keywords

  • Immobilization
  • Lipase
  • Pretreatment
  • Soybean oil

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology
  • Microbiology
  • Bioengineering

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