Optimization of Medium Components for p-glucosidase Production in Schizophyllum commune KUC9397 and Enzymatic Hydrolysis of Lignocellulosic Biomass

Young Min Lee, Hanbyul Lee, Jun Seok Kim, Jaejung Lee, Byoung Jun Ahn, Gyu Hyeok Kim, Jae-Jin Kim

Research output: Contribution to journalArticlepeer-review

14 Citations (Scopus)

Abstract

Medium components for β-glucosidase (BGL) production in Schizophyllum commune KUC9397 were optimized using a central composite design and response surface methodology. From the various medium components tested, cellulose, soy peptone, and thiamine HCl were selected as the optimal carbon, nitrogen, and vitamin sources, respectively. The highest BGL production was obtained with 2.96% cellulose, 2.30% soy peptone, and 0.11% thiamine HCl. BGL production in the optimized medium was increased 7.2-fold compared to production in an unoptimized medium. Crude enzyme preparation from S. commune KUC9397 was used to saccharify pretreated lignocellulosic biomass. The crude enzyme preparations showed statistically equal saccharification rates as Cellobiase, a commercial BGL. This finding indicates that crude enzymes produced by S. commune KUC9397 have good potential for application in cellulosic biomass conversion systems in place of Cellobiase.

Original languageEnglish
Pages (from-to)4358-4368
Number of pages11
JournalBioResources
Volume9
Issue number3
Publication statusPublished - 2014 Aug

Keywords

  • Optimization
  • Saccharification
  • Schizophyllum commune
  • ß-glucosidase

ASJC Scopus subject areas

  • Environmental Engineering
  • Bioengineering
  • Waste Management and Disposal

Fingerprint

Dive into the research topics of 'Optimization of Medium Components for p-glucosidase Production in Schizophyllum commune KUC9397 and Enzymatic Hydrolysis of Lignocellulosic Biomass'. Together they form a unique fingerprint.

Cite this