Optimization of medium composition for enhanced cellulase production by mutant Penicillium brasilianum KUEB15 using statistical method

Da Un Jung, Hah Young Yoo, Sung Bong Kim, Ja Hyun Lee, Chulhwan Park, Seung Wook Kim

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Penicillium brasilianum KUEB15 was utilized for cellulase production and medium optimization was investigated. The optimal compositions of the variables for maximum CMCase production by central composite rotatable design were 12.39. g/L of corn stover, 0.48. g/L of urea, and 6.12. g/L of ammonium sulfate. The predicted CMCase activity by the model was 1.207. Unit/ml, and the observed CMCase activity which was produced in optimal media was 1.180. Unit/ml. The activity of CMCase from P. brasilianum KUEB15, which was fermented in optimal media by RSM, was found to be 1.61-fold increased than that in the Mandel's medium (0.731. Unit/ml).

Original languageEnglish
Pages (from-to)145-150
Number of pages6
JournalJournal of Industrial and Engineering Chemistry
Volume25
DOIs
Publication statusPublished - 2015 May 25

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Cellulase
Statistical methods
Chemical analysis
Urea
Composite materials
Ammonium Sulfate
carboxymethylcellulase
Sulfates

Keywords

  • Cellulase
  • Central composite rotatable design
  • Optimization
  • Penicillium brasilianum
  • Plackett-Burman design

ASJC Scopus subject areas

  • Chemical Engineering(all)

Cite this

Optimization of medium composition for enhanced cellulase production by mutant Penicillium brasilianum KUEB15 using statistical method. / Jung, Da Un; Yoo, Hah Young; Kim, Sung Bong; Lee, Ja Hyun; Park, Chulhwan; Kim, Seung Wook.

In: Journal of Industrial and Engineering Chemistry, Vol. 25, 25.05.2015, p. 145-150.

Research output: Contribution to journalArticle

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AU - Park, Chulhwan

AU - Kim, Seung Wook

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