Optimization of medium composition for enhanced cellulase production by mutant Penicillium brasilianum KUEB15 using statistical method

Da Un Jung; Hah Young Yoo; Sung Bong Kim; Ja Hyun Lee; Chulhwan Park; Seung Wook Kim

doi:10.1016/j.jiec.2014.10.026

Optimization of medium composition for enhanced cellulase production by mutant Penicillium brasilianum KUEB15 using statistical method

Da Un Jung, Hah Young Yoo, Sung Bong Kim, Ja Hyun Lee, Chulhwan Park, Seung Wook Kim

Department of Chemical and Biological Engineering

Research output: Contribution to journal › Article › peer-review

30 Citations (Scopus)

Abstract

Penicillium brasilianum KUEB15 was utilized for cellulase production and medium optimization was investigated. The optimal compositions of the variables for maximum CMCase production by central composite rotatable design were 12.39. g/L of corn stover, 0.48. g/L of urea, and 6.12. g/L of ammonium sulfate. The predicted CMCase activity by the model was 1.207. Unit/ml, and the observed CMCase activity which was produced in optimal media was 1.180. Unit/ml. The activity of CMCase from P. brasilianum KUEB15, which was fermented in optimal media by RSM, was found to be 1.61-fold increased than that in the Mandel's medium (0.731. Unit/ml).

Original language	English
Pages (from-to)	145-150
Number of pages	6
Journal	Journal of Industrial and Engineering Chemistry
Volume	25
DOIs	https://doi.org/10.1016/j.jiec.2014.10.026
Publication status	Published - 2015 May 25

Keywords

Cellulase
Central composite rotatable design
Optimization
Penicillium brasilianum
Plackett-Burman design

ASJC Scopus subject areas

Chemical Engineering(all)

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10.1016/j.jiec.2014.10.026

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title = "Optimization of medium composition for enhanced cellulase production by mutant Penicillium brasilianum KUEB15 using statistical method",

abstract = "Penicillium brasilianum KUEB15 was utilized for cellulase production and medium optimization was investigated. The optimal compositions of the variables for maximum CMCase production by central composite rotatable design were 12.39. g/L of corn stover, 0.48. g/L of urea, and 6.12. g/L of ammonium sulfate. The predicted CMCase activity by the model was 1.207. Unit/ml, and the observed CMCase activity which was produced in optimal media was 1.180. Unit/ml. The activity of CMCase from P. brasilianum KUEB15, which was fermented in optimal media by RSM, was found to be 1.61-fold increased than that in the Mandel's medium (0.731. Unit/ml).",

keywords = "Cellulase, Central composite rotatable design, Optimization, Penicillium brasilianum, Plackett-Burman design",

author = "Jung, {Da Un} and Yoo, {Hah Young} and Kim, {Sung Bong} and Lee, {Ja Hyun} and Chulhwan Park and Kim, {Seung Wook}",

note = "Funding Information: This work was supported by the Advanced Biomass R&D Center (ABC-2011-0031360) of the Global Frontier Project funded by the Ministry of Science, ICT and Future Planning of Korea, the National Research Foundation of Korea Grant funded by the Korean Government (MSIP) (No. 2014R1A2A2A01007321 ), and Basic Science Research Program through the National Research Foundation of Korea funded by the Ministry of Science, ICT and Future Planning ( 2012M2B2A4029962 ). Publisher Copyright: {\textcopyright} 2014 The Korean Society of Industrial and Engineering Chemistry.",

year = "2015",

month = may,

day = "25",

doi = "10.1016/j.jiec.2014.10.026",

language = "English",

volume = "25",

pages = "145--150",

journal = "Journal of Industrial and Engineering Chemistry",

issn = "1226-086X",

publisher = "Korean Society of Industrial Engineering Chemistry",

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T1 - Optimization of medium composition for enhanced cellulase production by mutant Penicillium brasilianum KUEB15 using statistical method

AU - Jung, Da Un

AU - Yoo, Hah Young

AU - Kim, Sung Bong

AU - Lee, Ja Hyun

AU - Park, Chulhwan

AU - Kim, Seung Wook

N1 - Funding Information: This work was supported by the Advanced Biomass R&D Center (ABC-2011-0031360) of the Global Frontier Project funded by the Ministry of Science, ICT and Future Planning of Korea, the National Research Foundation of Korea Grant funded by the Korean Government (MSIP) (No. 2014R1A2A2A01007321 ), and Basic Science Research Program through the National Research Foundation of Korea funded by the Ministry of Science, ICT and Future Planning ( 2012M2B2A4029962 ). Publisher Copyright: © 2014 The Korean Society of Industrial and Engineering Chemistry.

PY - 2015/5/25

Y1 - 2015/5/25

N2 - Penicillium brasilianum KUEB15 was utilized for cellulase production and medium optimization was investigated. The optimal compositions of the variables for maximum CMCase production by central composite rotatable design were 12.39. g/L of corn stover, 0.48. g/L of urea, and 6.12. g/L of ammonium sulfate. The predicted CMCase activity by the model was 1.207. Unit/ml, and the observed CMCase activity which was produced in optimal media was 1.180. Unit/ml. The activity of CMCase from P. brasilianum KUEB15, which was fermented in optimal media by RSM, was found to be 1.61-fold increased than that in the Mandel's medium (0.731. Unit/ml).

AB - Penicillium brasilianum KUEB15 was utilized for cellulase production and medium optimization was investigated. The optimal compositions of the variables for maximum CMCase production by central composite rotatable design were 12.39. g/L of corn stover, 0.48. g/L of urea, and 6.12. g/L of ammonium sulfate. The predicted CMCase activity by the model was 1.207. Unit/ml, and the observed CMCase activity which was produced in optimal media was 1.180. Unit/ml. The activity of CMCase from P. brasilianum KUEB15, which was fermented in optimal media by RSM, was found to be 1.61-fold increased than that in the Mandel's medium (0.731. Unit/ml).

KW - Cellulase

KW - Central composite rotatable design

KW - Optimization

KW - Penicillium brasilianum

KW - Plackett-Burman design

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DO - 10.1016/j.jiec.2014.10.026

M3 - Article

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VL - 25

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EP - 150

JO - Journal of Industrial and Engineering Chemistry

JF - Journal of Industrial and Engineering Chemistry

SN - 1226-086X

ER -

Optimization of medium composition for enhanced cellulase production by mutant Penicillium brasilianum KUEB15 using statistical method

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