Abstract
Penicillium brasilianum KUEB15 was utilized for cellulase production and medium optimization was investigated. The optimal compositions of the variables for maximum CMCase production by central composite rotatable design were 12.39. g/L of corn stover, 0.48. g/L of urea, and 6.12. g/L of ammonium sulfate. The predicted CMCase activity by the model was 1.207. Unit/ml, and the observed CMCase activity which was produced in optimal media was 1.180. Unit/ml. The activity of CMCase from P. brasilianum KUEB15, which was fermented in optimal media by RSM, was found to be 1.61-fold increased than that in the Mandel's medium (0.731. Unit/ml).
| Original language | English |
|---|---|
| Pages (from-to) | 145-150 |
| Number of pages | 6 |
| Journal | Journal of Industrial and Engineering Chemistry |
| Volume | 25 |
| DOIs | |
| Publication status | Published - 2015 May 25 |
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Keywords
- Cellulase
- Central composite rotatable design
- Optimization
- Penicillium brasilianum
- Plackett-Burman design
ASJC Scopus subject areas
- Chemical Engineering(all)
Cite this
Optimization of medium composition for enhanced cellulase production by mutant Penicillium brasilianum KUEB15 using statistical method. / Jung, Da Un; Yoo, Hah Young; Kim, Sung Bong; Lee, Ja Hyun; Park, Chulhwan; Kim, Seung Wook.
In: Journal of Industrial and Engineering Chemistry, Vol. 25, 25.05.2015, p. 145-150.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Optimization of medium composition for enhanced cellulase production by mutant Penicillium brasilianum KUEB15 using statistical method
AU - Jung, Da Un
AU - Yoo, Hah Young
AU - Kim, Sung Bong
AU - Lee, Ja Hyun
AU - Park, Chulhwan
AU - Kim, Seung Wook
PY - 2015/5/25
Y1 - 2015/5/25
N2 - Penicillium brasilianum KUEB15 was utilized for cellulase production and medium optimization was investigated. The optimal compositions of the variables for maximum CMCase production by central composite rotatable design were 12.39. g/L of corn stover, 0.48. g/L of urea, and 6.12. g/L of ammonium sulfate. The predicted CMCase activity by the model was 1.207. Unit/ml, and the observed CMCase activity which was produced in optimal media was 1.180. Unit/ml. The activity of CMCase from P. brasilianum KUEB15, which was fermented in optimal media by RSM, was found to be 1.61-fold increased than that in the Mandel's medium (0.731. Unit/ml).
AB - Penicillium brasilianum KUEB15 was utilized for cellulase production and medium optimization was investigated. The optimal compositions of the variables for maximum CMCase production by central composite rotatable design were 12.39. g/L of corn stover, 0.48. g/L of urea, and 6.12. g/L of ammonium sulfate. The predicted CMCase activity by the model was 1.207. Unit/ml, and the observed CMCase activity which was produced in optimal media was 1.180. Unit/ml. The activity of CMCase from P. brasilianum KUEB15, which was fermented in optimal media by RSM, was found to be 1.61-fold increased than that in the Mandel's medium (0.731. Unit/ml).
KW - Cellulase
KW - Central composite rotatable design
KW - Optimization
KW - Penicillium brasilianum
KW - Plackett-Burman design
UR - http://www.scopus.com/inward/record.url?scp=84930063709&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84930063709&partnerID=8YFLogxK
U2 - 10.1016/j.jiec.2014.10.026
DO - 10.1016/j.jiec.2014.10.026
M3 - Article
VL - 25
SP - 145
EP - 150
JO - Journal of Industrial and Engineering Chemistry
JF - Journal of Industrial and Engineering Chemistry
SN - 1226-086X
ER -