Optimization of medium composition for enhanced cellulase production by mutant Penicillium brasilianum KUEB15 using statistical method

Da Un Jung; Hah Young Yoo; Sung Bong Kim; Ja Hyun Lee; Chulhwan Park; Seung Wook Kim

doi:10.1016/j.jiec.2014.10.026

Optimization of medium composition for enhanced cellulase production by mutant Penicillium brasilianum KUEB15 using statistical method

Da Un Jung, Hah Young Yoo, Sung Bong Kim, Ja Hyun Lee, Chulhwan Park, Seung Wook Kim

Department of Chemical and Biological Engineering

Research output: Contribution to journal › Article

20 Citations (Scopus)

Abstract

Penicillium brasilianum KUEB15 was utilized for cellulase production and medium optimization was investigated. The optimal compositions of the variables for maximum CMCase production by central composite rotatable design were 12.39. g/L of corn stover, 0.48. g/L of urea, and 6.12. g/L of ammonium sulfate. The predicted CMCase activity by the model was 1.207. Unit/ml, and the observed CMCase activity which was produced in optimal media was 1.180. Unit/ml. The activity of CMCase from P. brasilianum KUEB15, which was fermented in optimal media by RSM, was found to be 1.61-fold increased than that in the Mandel's medium (0.731. Unit/ml).

Original language	English
Pages (from-to)	145-150
Number of pages	6
Journal	Journal of Industrial and Engineering Chemistry
Volume	25
DOIs	https://doi.org/10.1016/j.jiec.2014.10.026
Publication status	Published - 2015 May 25

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Keywords

Cellulase
Central composite rotatable design
Optimization
Penicillium brasilianum
Plackett-Burman design

ASJC Scopus subject areas

Chemical Engineering(all)

Cite this

Jung, D. U., Yoo, H. Y., Kim, S. B., Lee, J. H., Park, C., & Kim, S. W. (2015). Optimization of medium composition for enhanced cellulase production by mutant Penicillium brasilianum KUEB15 using statistical method. Journal of Industrial and Engineering Chemistry, 25, 145-150. https://doi.org/10.1016/j.jiec.2014.10.026

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title = "Optimization of medium composition for enhanced cellulase production by mutant Penicillium brasilianum KUEB15 using statistical method",

abstract = "Penicillium brasilianum KUEB15 was utilized for cellulase production and medium optimization was investigated. The optimal compositions of the variables for maximum CMCase production by central composite rotatable design were 12.39. g/L of corn stover, 0.48. g/L of urea, and 6.12. g/L of ammonium sulfate. The predicted CMCase activity by the model was 1.207. Unit/ml, and the observed CMCase activity which was produced in optimal media was 1.180. Unit/ml. The activity of CMCase from P. brasilianum KUEB15, which was fermented in optimal media by RSM, was found to be 1.61-fold increased than that in the Mandel's medium (0.731. Unit/ml).",

keywords = "Cellulase, Central composite rotatable design, Optimization, Penicillium brasilianum, Plackett-Burman design",

author = "Jung, {Da Un} and Yoo, {Hah Young} and Kim, {Sung Bong} and Lee, {Ja Hyun} and Chulhwan Park and Kim, {Seung Wook}",

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AU - Jung, Da Un

AU - Yoo, Hah Young

AU - Kim, Sung Bong

AU - Lee, Ja Hyun

AU - Park, Chulhwan

AU - Kim, Seung Wook

PY - 2015/5/25

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N2 - Penicillium brasilianum KUEB15 was utilized for cellulase production and medium optimization was investigated. The optimal compositions of the variables for maximum CMCase production by central composite rotatable design were 12.39. g/L of corn stover, 0.48. g/L of urea, and 6.12. g/L of ammonium sulfate. The predicted CMCase activity by the model was 1.207. Unit/ml, and the observed CMCase activity which was produced in optimal media was 1.180. Unit/ml. The activity of CMCase from P. brasilianum KUEB15, which was fermented in optimal media by RSM, was found to be 1.61-fold increased than that in the Mandel's medium (0.731. Unit/ml).

AB - Penicillium brasilianum KUEB15 was utilized for cellulase production and medium optimization was investigated. The optimal compositions of the variables for maximum CMCase production by central composite rotatable design were 12.39. g/L of corn stover, 0.48. g/L of urea, and 6.12. g/L of ammonium sulfate. The predicted CMCase activity by the model was 1.207. Unit/ml, and the observed CMCase activity which was produced in optimal media was 1.180. Unit/ml. The activity of CMCase from P. brasilianum KUEB15, which was fermented in optimal media by RSM, was found to be 1.61-fold increased than that in the Mandel's medium (0.731. Unit/ml).

KW - Cellulase

KW - Central composite rotatable design

KW - Optimization

KW - Penicillium brasilianum

KW - Plackett-Burman design

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10.1016/j.jiec.2014.10.026