Optimization of scaffold for a successful hydrogel-seeding method for vascular tissue engineering

In Su Park, Sang Heon Kim, Jeong Woo Han, Young Gun Ko, Eunna Chung, Soo Hyun Kim

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Porosity and pore size are needed for successful cell seeding and proliferation into porous scaffolds. This study was focused on a hydrogel-seeding method to improve cell adhesion and proliferation in tubular porous scaffolds for vascular grafts application. Tubular scaffolds were fabricated from a biodegradable elastic polymer, poly(L-lactide-co-ε- caprolactone) (PLCL) (50:50, Mn 1.58×105), by an extrusion-particulate leaching method. Vascular smooth muscle cells (VSMCs) were dispersed in collagen hydrogel and then seeded into the tubular PLCL scaffolds having various pore sizes, 50-100 urn, 100-200 μm, and 300-500 μm, respectively. As a result, the efficiency of cell adhesion and proliferation was dependent on the pore size of the scaffolds. Especially, the cell proliferation efficiency was improved by using the hydrogel-seeding method as compared with by using a previously established method. In summary, this study demonstrates that the efficiency of cell adhesion and proliferation was dependent on the pore size of the scaffolds in the hydrogel-seeding method.

Original languageEnglish
Pages (from-to)333-336
Number of pages4
JournalKey Engineering Materials
Volume342-343
Publication statusPublished - 2007 Apr 16
Externally publishedYes

Fingerprint

Hydrogel
Cell proliferation
Tissue engineering
Hydrogels
Scaffolds
Pore size
Cell adhesion
Scaffolds (biology)
Collagen
Grafts
Leaching
Extrusion
Muscle
Polymers
Porosity
Cells

Keywords

  • Collagen
  • Hydrogel
  • Pore size
  • Smooth muscle cells
  • Vascular tissue engineering

ASJC Scopus subject areas

  • Ceramics and Composites
  • Chemical Engineering (miscellaneous)

Cite this

Park, I. S., Kim, S. H., Han, J. W., Ko, Y. G., Chung, E., & Kim, S. H. (2007). Optimization of scaffold for a successful hydrogel-seeding method for vascular tissue engineering. Key Engineering Materials, 342-343, 333-336.

Optimization of scaffold for a successful hydrogel-seeding method for vascular tissue engineering. / Park, In Su; Kim, Sang Heon; Han, Jeong Woo; Ko, Young Gun; Chung, Eunna; Kim, Soo Hyun.

In: Key Engineering Materials, Vol. 342-343, 16.04.2007, p. 333-336.

Research output: Contribution to journalArticle

Park, IS, Kim, SH, Han, JW, Ko, YG, Chung, E & Kim, SH 2007, 'Optimization of scaffold for a successful hydrogel-seeding method for vascular tissue engineering', Key Engineering Materials, vol. 342-343, pp. 333-336.
Park, In Su ; Kim, Sang Heon ; Han, Jeong Woo ; Ko, Young Gun ; Chung, Eunna ; Kim, Soo Hyun. / Optimization of scaffold for a successful hydrogel-seeding method for vascular tissue engineering. In: Key Engineering Materials. 2007 ; Vol. 342-343. pp. 333-336.
@article{1b7933c326f14bb3b688341278432f05,
title = "Optimization of scaffold for a successful hydrogel-seeding method for vascular tissue engineering",
abstract = "Porosity and pore size are needed for successful cell seeding and proliferation into porous scaffolds. This study was focused on a hydrogel-seeding method to improve cell adhesion and proliferation in tubular porous scaffolds for vascular grafts application. Tubular scaffolds were fabricated from a biodegradable elastic polymer, poly(L-lactide-co-ε- caprolactone) (PLCL) (50:50, Mn 1.58×105), by an extrusion-particulate leaching method. Vascular smooth muscle cells (VSMCs) were dispersed in collagen hydrogel and then seeded into the tubular PLCL scaffolds having various pore sizes, 50-100 urn, 100-200 μm, and 300-500 μm, respectively. As a result, the efficiency of cell adhesion and proliferation was dependent on the pore size of the scaffolds. Especially, the cell proliferation efficiency was improved by using the hydrogel-seeding method as compared with by using a previously established method. In summary, this study demonstrates that the efficiency of cell adhesion and proliferation was dependent on the pore size of the scaffolds in the hydrogel-seeding method.",
keywords = "Collagen, Hydrogel, Pore size, Smooth muscle cells, Vascular tissue engineering",
author = "Park, {In Su} and Kim, {Sang Heon} and Han, {Jeong Woo} and Ko, {Young Gun} and Eunna Chung and Kim, {Soo Hyun}",
year = "2007",
month = "4",
day = "16",
language = "English",
volume = "342-343",
pages = "333--336",
journal = "Key Engineering Materials",
issn = "1013-9826",
publisher = "Trans Tech Publications",

}

TY - JOUR

T1 - Optimization of scaffold for a successful hydrogel-seeding method for vascular tissue engineering

AU - Park, In Su

AU - Kim, Sang Heon

AU - Han, Jeong Woo

AU - Ko, Young Gun

AU - Chung, Eunna

AU - Kim, Soo Hyun

PY - 2007/4/16

Y1 - 2007/4/16

N2 - Porosity and pore size are needed for successful cell seeding and proliferation into porous scaffolds. This study was focused on a hydrogel-seeding method to improve cell adhesion and proliferation in tubular porous scaffolds for vascular grafts application. Tubular scaffolds were fabricated from a biodegradable elastic polymer, poly(L-lactide-co-ε- caprolactone) (PLCL) (50:50, Mn 1.58×105), by an extrusion-particulate leaching method. Vascular smooth muscle cells (VSMCs) were dispersed in collagen hydrogel and then seeded into the tubular PLCL scaffolds having various pore sizes, 50-100 urn, 100-200 μm, and 300-500 μm, respectively. As a result, the efficiency of cell adhesion and proliferation was dependent on the pore size of the scaffolds. Especially, the cell proliferation efficiency was improved by using the hydrogel-seeding method as compared with by using a previously established method. In summary, this study demonstrates that the efficiency of cell adhesion and proliferation was dependent on the pore size of the scaffolds in the hydrogel-seeding method.

AB - Porosity and pore size are needed for successful cell seeding and proliferation into porous scaffolds. This study was focused on a hydrogel-seeding method to improve cell adhesion and proliferation in tubular porous scaffolds for vascular grafts application. Tubular scaffolds were fabricated from a biodegradable elastic polymer, poly(L-lactide-co-ε- caprolactone) (PLCL) (50:50, Mn 1.58×105), by an extrusion-particulate leaching method. Vascular smooth muscle cells (VSMCs) were dispersed in collagen hydrogel and then seeded into the tubular PLCL scaffolds having various pore sizes, 50-100 urn, 100-200 μm, and 300-500 μm, respectively. As a result, the efficiency of cell adhesion and proliferation was dependent on the pore size of the scaffolds. Especially, the cell proliferation efficiency was improved by using the hydrogel-seeding method as compared with by using a previously established method. In summary, this study demonstrates that the efficiency of cell adhesion and proliferation was dependent on the pore size of the scaffolds in the hydrogel-seeding method.

KW - Collagen

KW - Hydrogel

KW - Pore size

KW - Smooth muscle cells

KW - Vascular tissue engineering

UR - http://www.scopus.com/inward/record.url?scp=34147158990&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34147158990&partnerID=8YFLogxK

M3 - Article

VL - 342-343

SP - 333

EP - 336

JO - Key Engineering Materials

JF - Key Engineering Materials

SN - 1013-9826

ER -