Optimization of scaffold for a successful hydrogel-seeding method for vascular tissue engineering

In Su Park, Sang Heon Kim, Jeong Woo Han, Young Gun Ko, Eunna Chung, Soo Hyun Kim

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Porosity and pore size are needed for successful cell seeding and proliferation into porous scaffolds. This study was focused on a hydrogel-seeding method to improve cell adhesion and proliferation in tubular porous scaffolds for vascular grafts application. Tubular scaffolds were fabricated from a biodegradable elastic polymer, poly(L-lactide-co-ε- caprolactone) (PLCL) (50:50, Mn 1.58×105), by an extrusion-particulate leaching method. Vascular smooth muscle cells (VSMCs) were dispersed in collagen hydrogel and then seeded into the tubular PLCL scaffolds having various pore sizes, 50-100 urn, 100-200 μm, and 300-500 μm, respectively. As a result, the efficiency of cell adhesion and proliferation was dependent on the pore size of the scaffolds. Especially, the cell proliferation efficiency was improved by using the hydrogel-seeding method as compared with by using a previously established method. In summary, this study demonstrates that the efficiency of cell adhesion and proliferation was dependent on the pore size of the scaffolds in the hydrogel-seeding method.

Original languageEnglish
Pages (from-to)333-336
Number of pages4
JournalKey Engineering Materials
Volume342-343
DOIs
Publication statusPublished - 2007

Keywords

  • Collagen
  • Hydrogel
  • Pore size
  • Smooth muscle cells
  • Vascular tissue engineering

ASJC Scopus subject areas

  • Materials Science(all)
  • Mechanics of Materials
  • Mechanical Engineering

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