Optimization of synergism of a recombinant auxiliary activity 9 from Chaetomium globosum with cellulase in cellulose hydrolysis

In Jung Kim, Ki Hyun Nam, Eun Ju Yun, Sooah Kim, Hak Jin Youn, Hee Jin Lee, In-Geol Choi, Kyoung Heon Kim

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Auxiliary activity family 9 (AA9, formerly known as glycoside hydrolase family 61 or polysaccharide monooxygenase) is a group of fungal proteins that were recently found to have a significant synergism with cellulase in cellulose hydrolysis via the oxidative cleavage of glycosidic bonds of cellulose chains. In this study, we report the active expression of a recombinant fungal AA9 from Chaetomium globosum (CgAA9) in a bacterial host, Escherichia coli, and the optimization of its synergistic activity in cellulose hydrolysis by using cellulase. The recombinant CgAA9 (0.9 mg/g cellulose) exhibited 1.7-fold synergism in the hydrolysis of Avicel when incubated with 0.9 filter paper units of Celluclast 1.5 L/g cellulose. The first study of the active expression of AA9 using a bacterial host and its synergistic optimization could be useful for the industrial application of AA9 for the saccharification of lignocellulose.

Original languageEnglish
Pages (from-to)8537-8547
Number of pages11
JournalApplied Microbiology and Biotechnology
Volume99
Issue number20
DOIs
Publication statusPublished - 2015 Oct 22

Fingerprint

Chaetomium
Cellulase
Cellulose
Hydrolysis
Fungal Proteins
Glycoside Hydrolases
Mixed Function Oxygenases
Polysaccharides
Escherichia coli

Keywords

  • AA9
  • Cellulase
  • Cellulose hydrolysis
  • Chaetomium globosum
  • GH61
  • Synergism

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

Cite this

Optimization of synergism of a recombinant auxiliary activity 9 from Chaetomium globosum with cellulase in cellulose hydrolysis. / Kim, In Jung; Nam, Ki Hyun; Yun, Eun Ju; Kim, Sooah; Youn, Hak Jin; Lee, Hee Jin; Choi, In-Geol; Kim, Kyoung Heon.

In: Applied Microbiology and Biotechnology, Vol. 99, No. 20, 22.10.2015, p. 8537-8547.

Research output: Contribution to journalArticle

Kim, In Jung ; Nam, Ki Hyun ; Yun, Eun Ju ; Kim, Sooah ; Youn, Hak Jin ; Lee, Hee Jin ; Choi, In-Geol ; Kim, Kyoung Heon. / Optimization of synergism of a recombinant auxiliary activity 9 from Chaetomium globosum with cellulase in cellulose hydrolysis. In: Applied Microbiology and Biotechnology. 2015 ; Vol. 99, No. 20. pp. 8537-8547.
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