Optimization of the process for biodiesel production using a mixture of immobilized Rhizopus oryzae and Candida rugosa lipases

Jong Ho Lee, Dong Hwan Lee, Jung Soo Lim, Byung Hwan Um, Chulhwan Park, Seong Woo Kang, Seung Wook Kim

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

In this study, the enzymatic process for biodiesel production was optimized using a mixture of immobilized Rhizopus oryzae and Candida rugosa lipases. The optimal temperature and agitation speed for biodiesel production were 45°C and 300 rpm, respectively. The optimal ratio of R. oryzae and C. rugosa lipases in the mixture was 3:1 (w:w). When 3 mmol of methanol was the initial reaction medium and 3 mmol of methanol was added every 1.5 h during biodiesel production, biodiesel conversion was over 98% at 4 h. In addition, when the immobilized lipase mixture was reused, biodiesel conversion exceeded 80% after 5 reuses.

Original languageEnglish
Pages (from-to)1927-1931
Number of pages5
JournalJournal of microbiology and biotechnology
Volume18
Issue number12
DOIs
Publication statusPublished - 2008 Dec 28

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Keywords

  • Biodiesel
  • Candida rugosa lipase
  • Immobilization
  • Rhizopus oryzae lipase

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

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