Optofluidic Surface Enhanced Raman Scattering based detection of single nucleotide polymorphism

Yun Suk Huh, Adam J. Lowe, Aram J. Chung, Bernardo Cordovez, Aaron D. Strickland, Carl A. Batt, David Erickson

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Single nucleotide polymorphisms (SNPs) have become key indicators of genetic disease, allelic variation associated with cancer progression, and pharmcogenomics. The ability rapidly screen for SNPs will have a profound impact on a number of applications, most notably personalized medicine. Here we demonstrate a new approach to SNP detection through the application of Surface Enhanced Raman Scattering (SERS) to the Ligase Detection Reaction (LDR). The reaction is implemented in an electrokinetically active microfluidic device that enables physical concentration of the reaction products for enhanced detection sensitivity and quantization. In addition, we present LDR-SERS multiplex SNP detection in the KRas oncogene and the direct correlation of diagnostic peak intensity to SNP concentration in mixed genotype samples.

Original languageEnglish
Title of host publicationProceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PublisherChemical and Biological Microsystems Society
Pages281-283
Number of pages3
ISBN (Print)9780979806421
Publication statusPublished - 2009
Externally publishedYes
Event13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009 - Jeju, Korea, Republic of
Duration: 2009 Nov 12009 Nov 5

Publication series

NameProceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences

Other

Other13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009
CountryKorea, Republic of
CityJeju
Period09/11/109/11/5

Keywords

  • Ligase detection reaction (LDR)
  • Optofluidic device
  • Single nucleotide polymorphism (SNP)
  • Surface-enhanced Raman scattering (SERS)

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering

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  • Cite this

    Huh, Y. S., Lowe, A. J., Chung, A. J., Cordovez, B., Strickland, A. D., Batt, C. A., & Erickson, D. (2009). Optofluidic Surface Enhanced Raman Scattering based detection of single nucleotide polymorphism. In Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences (pp. 281-283). (Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences). Chemical and Biological Microsystems Society.