Optogenetic manipulation of neural activity in freely moving Caenorhabditis elegans

Andrew M. Leifer, Christopher Fang-Yen, Marc Gershow, Mark J. Alkema, Aravinthan D T Samuel

Research output: Contribution to journalArticle

177 Citations (Scopus)

Abstract

We present an optogenetic illumination system capable of real-time light delivery with high spatial resolution to specified targets in freely moving Caenorhabditis elegans. A tracking microscope records the motion of an unrestrained worm expressing channelrhodopsin-2 or halorhodopsin in specific cell types. Image processing software analyzes the worm's position in each video frame, rapidly estimates the locations of targeted cells and instructs a digital micromirror device to illuminate targeted cells with laser light of the appropriate wavelengths to stimulate or inhibit activity. Because each cell in an unrestrained worm is a rapidly moving target, our system operates at high speed (∼50 frames per second) to provide high spatial resolution (∼30 μm). To test the accuracy, flexibility and utility of our system, we performed optogenetic analyses of the worm motor circuit, egg-laying circuit and mechanosensory circuits that have not been possible with previous methods.

Original languageEnglish
Pages (from-to)147-152
Number of pages6
JournalNature Methods
Volume8
Issue number2
DOIs
Publication statusPublished - 2011 Feb 1
Externally publishedYes

Fingerprint

Optogenetics
Caenorhabditis elegans
Networks (circuits)
Halorhodopsins
Digital devices
Light
Computer Systems
Image processing
Microscopes
Lighting
Ovum
Wavelength
Lasers
Software
Equipment and Supplies

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Biology
  • Biochemistry
  • Cell Biology

Cite this

Leifer, A. M., Fang-Yen, C., Gershow, M., Alkema, M. J., & Samuel, A. D. T. (2011). Optogenetic manipulation of neural activity in freely moving Caenorhabditis elegans. Nature Methods, 8(2), 147-152. https://doi.org/10.1038/nmeth.1554

Optogenetic manipulation of neural activity in freely moving Caenorhabditis elegans. / Leifer, Andrew M.; Fang-Yen, Christopher; Gershow, Marc; Alkema, Mark J.; Samuel, Aravinthan D T.

In: Nature Methods, Vol. 8, No. 2, 01.02.2011, p. 147-152.

Research output: Contribution to journalArticle

Leifer, AM, Fang-Yen, C, Gershow, M, Alkema, MJ & Samuel, ADT 2011, 'Optogenetic manipulation of neural activity in freely moving Caenorhabditis elegans', Nature Methods, vol. 8, no. 2, pp. 147-152. https://doi.org/10.1038/nmeth.1554
Leifer, Andrew M. ; Fang-Yen, Christopher ; Gershow, Marc ; Alkema, Mark J. ; Samuel, Aravinthan D T. / Optogenetic manipulation of neural activity in freely moving Caenorhabditis elegans. In: Nature Methods. 2011 ; Vol. 8, No. 2. pp. 147-152.
@article{1a4a4e28c7a5449bb50a2f17a8afc1ba,
title = "Optogenetic manipulation of neural activity in freely moving Caenorhabditis elegans",
abstract = "We present an optogenetic illumination system capable of real-time light delivery with high spatial resolution to specified targets in freely moving Caenorhabditis elegans. A tracking microscope records the motion of an unrestrained worm expressing channelrhodopsin-2 or halorhodopsin in specific cell types. Image processing software analyzes the worm's position in each video frame, rapidly estimates the locations of targeted cells and instructs a digital micromirror device to illuminate targeted cells with laser light of the appropriate wavelengths to stimulate or inhibit activity. Because each cell in an unrestrained worm is a rapidly moving target, our system operates at high speed (∼50 frames per second) to provide high spatial resolution (∼30 μm). To test the accuracy, flexibility and utility of our system, we performed optogenetic analyses of the worm motor circuit, egg-laying circuit and mechanosensory circuits that have not been possible with previous methods.",
author = "Leifer, {Andrew M.} and Christopher Fang-Yen and Marc Gershow and Alkema, {Mark J.} and Samuel, {Aravinthan D T}",
year = "2011",
month = "2",
day = "1",
doi = "10.1038/nmeth.1554",
language = "English",
volume = "8",
pages = "147--152",
journal = "PLoS Medicine",
issn = "1549-1277",
publisher = "Nature Publishing Group",
number = "2",

}

TY - JOUR

T1 - Optogenetic manipulation of neural activity in freely moving Caenorhabditis elegans

AU - Leifer, Andrew M.

AU - Fang-Yen, Christopher

AU - Gershow, Marc

AU - Alkema, Mark J.

AU - Samuel, Aravinthan D T

PY - 2011/2/1

Y1 - 2011/2/1

N2 - We present an optogenetic illumination system capable of real-time light delivery with high spatial resolution to specified targets in freely moving Caenorhabditis elegans. A tracking microscope records the motion of an unrestrained worm expressing channelrhodopsin-2 or halorhodopsin in specific cell types. Image processing software analyzes the worm's position in each video frame, rapidly estimates the locations of targeted cells and instructs a digital micromirror device to illuminate targeted cells with laser light of the appropriate wavelengths to stimulate or inhibit activity. Because each cell in an unrestrained worm is a rapidly moving target, our system operates at high speed (∼50 frames per second) to provide high spatial resolution (∼30 μm). To test the accuracy, flexibility and utility of our system, we performed optogenetic analyses of the worm motor circuit, egg-laying circuit and mechanosensory circuits that have not been possible with previous methods.

AB - We present an optogenetic illumination system capable of real-time light delivery with high spatial resolution to specified targets in freely moving Caenorhabditis elegans. A tracking microscope records the motion of an unrestrained worm expressing channelrhodopsin-2 or halorhodopsin in specific cell types. Image processing software analyzes the worm's position in each video frame, rapidly estimates the locations of targeted cells and instructs a digital micromirror device to illuminate targeted cells with laser light of the appropriate wavelengths to stimulate or inhibit activity. Because each cell in an unrestrained worm is a rapidly moving target, our system operates at high speed (∼50 frames per second) to provide high spatial resolution (∼30 μm). To test the accuracy, flexibility and utility of our system, we performed optogenetic analyses of the worm motor circuit, egg-laying circuit and mechanosensory circuits that have not been possible with previous methods.

UR - http://www.scopus.com/inward/record.url?scp=79551556801&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79551556801&partnerID=8YFLogxK

U2 - 10.1038/nmeth.1554

DO - 10.1038/nmeth.1554

M3 - Article

VL - 8

SP - 147

EP - 152

JO - PLoS Medicine

JF - PLoS Medicine

SN - 1549-1277

IS - 2

ER -