Opuntia humifusa Partitioned Extracts Inhibit the Growth of U87MG Human Glioblastoma Cells

Sahng Wook Hahm, Jieun Park, Yong Suk Son

Research output: Contribution to journalArticlepeer-review

32 Citations (Scopus)

Abstract

Opuntia humifusa, a member of the Cactaceae family widely distributed in the southern regions of the Korean peninsula, has potential bioactive functions and medicinal benefits. In the present study, we investigated the effect of hexane, ethyl acetate extracts and water partitioned fraction of O. humifusa on proliferation, G1 arrest and apoptosis in U87MG human glioblastoma cells. Glioblastoma cellular proliferation was evaluated using the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay, and the effects of O. humifusa partitioned extracts on cell cycle and apoptosis were analyzed by flow cytometry. Our results revealed that when U87MG cells were treated with hexane extracts and water partitioned fraction of O. humifusa, the number of viable cells decreased in a concentration-dependent manner. In addition, water partitioned fractions of O. humifusa induced G1 arrest and non-apoptotic cell death as well as significant increases in ROS production in U87MG cells. In conclusion, water partitioned fractions of O. humifusa induce G1 arrest and inhibit U87MG human glioblastoma cell proliferation.

Original languageEnglish
Pages (from-to)247-252
Number of pages6
JournalPlant Foods for Human Nutrition
Volume65
Issue number3
DOIs
Publication statusPublished - 2010

Keywords

  • Antiproliferative activity
  • G1 arrest
  • Opuntia humifusa
  • U87MG
  • Water partitioned fractions

ASJC Scopus subject areas

  • Food Science
  • Chemistry (miscellaneous)

Fingerprint Dive into the research topics of 'Opuntia humifusa Partitioned Extracts Inhibit the Growth of U87MG Human Glioblastoma Cells'. Together they form a unique fingerprint.

Cite this