Opuntia humifusa Partitioned Extracts Inhibit the Growth of U87MG Human Glioblastoma Cells

Sahng Wook Hahm, Jieun Park, Yong-Suk Son

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Opuntia humifusa, a member of the Cactaceae family widely distributed in the southern regions of the Korean peninsula, has potential bioactive functions and medicinal benefits. In the present study, we investigated the effect of hexane, ethyl acetate extracts and water partitioned fraction of O. humifusa on proliferation, G1 arrest and apoptosis in U87MG human glioblastoma cells. Glioblastoma cellular proliferation was evaluated using the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay, and the effects of O. humifusa partitioned extracts on cell cycle and apoptosis were analyzed by flow cytometry. Our results revealed that when U87MG cells were treated with hexane extracts and water partitioned fraction of O. humifusa, the number of viable cells decreased in a concentration-dependent manner. In addition, water partitioned fractions of O. humifusa induced G1 arrest and non-apoptotic cell death as well as significant increases in ROS production in U87MG cells. In conclusion, water partitioned fractions of O. humifusa induce G1 arrest and inhibit U87MG human glioblastoma cell proliferation.

Original languageEnglish
Pages (from-to)247-252
Number of pages6
JournalPlant Foods for Human Nutrition
Volume65
Issue number3
DOIs
Publication statusPublished - 2010 Sep 7

Fingerprint

Opuntia humifusa
Opuntia
human growth
Glioblastoma
Water
extracts
Hexanes
Growth
Cell Proliferation
Cactaceae
cells
Apoptosis
hexane
Flow cytometry
cell proliferation
Cell proliferation
Cell death
apoptosis
water
Assays

Keywords

  • Antiproliferative activity
  • G1 arrest
  • Opuntia humifusa
  • U87MG
  • Water partitioned fractions

ASJC Scopus subject areas

  • Food Science
  • Chemistry (miscellaneous)

Cite this

Opuntia humifusa Partitioned Extracts Inhibit the Growth of U87MG Human Glioblastoma Cells. / Hahm, Sahng Wook; Park, Jieun; Son, Yong-Suk.

In: Plant Foods for Human Nutrition, Vol. 65, No. 3, 07.09.2010, p. 247-252.

Research output: Contribution to journalArticle

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