ORF2 protein of porcine circovirus type 2 promotes phagocytic activity of porcine macrophages by inhibiting proteasomal degradation of complement component 1, q subcomponent binding protein (C1QBP) through physical interaction

Chang Yong Choi, Hae Na Oh, Suk Jun Lee, Taehoon Chun

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Defining how each ORF of porcine circovirus type 2 (PCV2) manipulates the host immune system may be helpful to understand the disease progression of post-weaning multisystemic wasting syndrome. In this study, we demonstrated a direct interaction between the PCV2 ORF2 and complement component 1, q subcomponent binding protein (C1QBP) within the cytoplasm of host macrophages. The physical interaction between PCV2 ORF2 and C1QBP inhibited ubiquitin-mediated proteasomal degradation of C1QBP in macrophages. Increased stability of C1QBP by the interaction with PCV2 ORF2 further enhanced the phagocytic activity of porcine macrophages through the phosphoinositol 3-kinase signalling pathway. This may explain the molecular basis of how PCV2 ORF2 enhances the phagocytic activity of host macrophages.

Original languageEnglish
Pages (from-to)3294-3301
Number of pages8
JournalJournal of General Virology
Volume96
Issue number11
DOIs
Publication statusPublished - 2015 Nov 1

ASJC Scopus subject areas

  • Virology

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