Organelle-selective di-(2-picolyl)amine-appended water-soluble fluorescent sensors for Cu(II): Synthesis, photophysical and in vitro studies

Yun Hak Lee, Peterts Verwilst, Nayoung Park, Joung Hae Lee, Jong Seung Kim

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

A novel water-soluble fluorescent naphthalimide derivative as a sensor for Cu(II) has been synthesized. The sensor, conjugated with DPA (as the Cu(II)-binding moiety) and TPP (as the mitochondrial-targeting moiety) was further decorated with a galactose unit at the fluorophore's terminal using the click reaction. Although confocal fluorescence imaging revealed that probe 1 was not localized in mitochondria, and was neither quenched under Cu-overloading conditions, probe 1 did exhibit a high selectivity for Cu(II) ions over various metal ions in HEPESbuffered solutions. Furthermore the fluorescence was dramatically quenched upon the addition 1 equivalent of Cu(II), and the response was stable in the range of pH 6-10.

Original languageEnglish
Pages (from-to)109-116
Number of pages8
JournalJournal of Inclusion Phenomena and Macrocyclic Chemistry
Volume82
Issue number1
DOIs
Publication statusPublished - 2015 Jun 1

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Keywords

  • Cell-imaging
  • Copper ions
  • Fluorescence
  • Subcellular environment

ASJC Scopus subject areas

  • Condensed Matter Physics
  • Food Science
  • Chemistry(all)

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