Organization of the presynaptic active zone by ERC2/CAST1-dependent clustering of the tandem PDZ protein syntenin-1

Jaewon Ko, Chan Yoon, Giovanni Piccoli, Sun Chung Hye, Karam Kim, Jae Ran Lee, Woo Lee Hyun, Hyun Kim, Carlo Sala, Eunjoon Kim

Research output: Contribution to journalArticlepeer-review

33 Citations (Scopus)

Abstract

Presynaptic active zones contain a cytoskeletal matrix called the CAZ, which is thought to play a critical role in the regulation of active zone formation and neurotransmitter release. Recent studies have identified several CAZ components, but little is known about how they contribute to the molecular organization of active zones. Here, we report a novel PDZ [postsynaptic density-95/Discs large/zona occludens-1] interaction between the CAZ protein ERC2/CAST1 and the tandem PDZ protein syntenin-1, which is known to associate with diverse synaptic proteins, including glutamate receptor subunits, SynCAM, and β-neurexin. This interaction promotes the localization of syntenin-1 at presynaptic ERC2 clusters. In addition to the PDZ interaction, multimerization of both ERC2 and syntenin-1 mediates syntenin-1 clustering. These results suggest that ERC2 promotes presynaptic syntenin-1 clustering by two distinct mechanisms and that syntenin-1 may contribute to the molecular organization of active zones by linking ERC2 and other CAZ components to diverse syntenin-1-associated synaptic proteins.

Original languageEnglish
Pages (from-to)963-970
Number of pages8
JournalJournal of Neuroscience
Volume26
Issue number3
DOIs
Publication statusPublished - 2006 Jan 18

Keywords

  • CAST
  • ERC
  • Multimerization
  • PDZ
  • Presynaptic regulation
  • Syntenin

ASJC Scopus subject areas

  • Neuroscience(all)

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