Organotypic slice culture of the hypothalamic paraventricular nucleus of rat

Seong Cho Eun, Yeong Lee So, Jae-Yong Park, Seong Geun Hong, Dong Ryu Pan

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Organotypic slice cultures have been developed as an alternative to acute brain slices because the neuronal viability and synaptic connectivity in these cultures can be preserved well for a prolonged period of time. This study evaluated a stationary organotypic slice culture developed for the hypothalamic paraventricular nucleus (PVN) of rat. The results showed that the slice cultures maintain the typical shape of the nucleus, the immunocytochemical signals for oxytocin, vasopressin, and corticotropin-releasing hormone, and the electrophysiological properties of PVN neurons for up to 3 weeks in vitro. The PVN neurons in the culture expressed the green fluorescent protein gene that had been delivered by the adenoviral vectors. The results indicate that the cultured slices preserve the properties of the PVN neurons, and can be used in long-term studies on these neurons in vitro.

Original languageEnglish
Pages (from-to)15-20
Number of pages6
JournalJournal of Veterinary Science
Volume8
Issue number1
Publication statusPublished - 2007 Mar 1
Externally publishedYes

Keywords

  • Electrophysiology
  • Green fluorescent protein
  • Immunocytochemistry
  • Organotypic slice culture
  • Paraventricular nucleus

ASJC Scopus subject areas

  • veterinary(all)

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