OsCO3, a CONSTANS-LIKE gene, controls flowering by negatively regulating the expression of FT-like genes under SD conditions in rice

Soon Kap Kim, Choong Hyo Yun, Jeong Hwan Lee, Yun Hee Jang, Hyo Young Park, Jeong Kook Kim

Research output: Contribution to journalArticle

73 Citations (Scopus)

Abstract

The photoperiod is an important environmental stress that determines flowering time. The CONSTANS (CO) and Heading date 1 (Hd1) genes are known to be central integrators of the photoperiod pathway in Arabidopsis and rice, respectively. Although they are both members of the CONSTANS-LIKE (COL) family and have two B-boxes and a CCT domain, rice also possesses novel COL genes that are not found in Arabidopsis. Here, we demonstrate that a novel COL gene, OsCO3, containing a single B-box and a CCT domain, modulates photoperiodic flowering in rice. The circadian expression pattern of OsCO3 mRNA oscillated in a different phase from Hd1 and was similar to that of OsCO3 pre-mRNA, suggesting that the diurnal expression pattern of OsCO3 transcripts may be regulated at the transcriptional level. Overexpression of OsCO3 specifically caused late flowering under short day (SD) conditions relative to wild-type rice plants. The expression of Hd3a and FTL decreased in these transgenic plants, whereas the expression of Hd1, Early heading date 1 (Ehd1), OsMADS51, and OsMADS50 did not significantly change. Our results suggest that OsCO3 primarily controls flowering time under SD conditions by negatively regulating Hd3a and FTL expression, independent of the SD-promotion pathway.

Original languageEnglish
Pages (from-to)355-365
Number of pages11
JournalPlanta
Volume228
Issue number2
DOIs
Publication statusPublished - 2008 Jul 1

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heading
flowering
rice
Photoperiod
Arabidopsis
Genes
genes
photoperiod
Genetically Modified Plants
RNA Precursors
transgenic plants
Messenger RNA
Oryza

Keywords

  • Floral repressor
  • OsCO3
  • Photoperiod
  • Single B-box

ASJC Scopus subject areas

  • Plant Science

Cite this

OsCO3, a CONSTANS-LIKE gene, controls flowering by negatively regulating the expression of FT-like genes under SD conditions in rice. / Kim, Soon Kap; Yun, Choong Hyo; Lee, Jeong Hwan; Jang, Yun Hee; Park, Hyo Young; Kim, Jeong Kook.

In: Planta, Vol. 228, No. 2, 01.07.2008, p. 355-365.

Research output: Contribution to journalArticle

Kim, Soon Kap ; Yun, Choong Hyo ; Lee, Jeong Hwan ; Jang, Yun Hee ; Park, Hyo Young ; Kim, Jeong Kook. / OsCO3, a CONSTANS-LIKE gene, controls flowering by negatively regulating the expression of FT-like genes under SD conditions in rice. In: Planta. 2008 ; Vol. 228, No. 2. pp. 355-365.
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