Abstract
The FCA gene, which is a component of the autonomous pathway that regulates flowering time, is an important example of how alternative processing can control plant development. We have previously characterized the FCA homolog, OsFCA, from a japonica-type rice cultivar and demonstrated that the polyadenylation site within intron 3, which can generate non-functional FCA-β, was conserved in rice. In this study, we detected five alternatively processed variants of OsFCA pre-mRNA, four of which were equivalents of FCA-α, -β, -γ, and -δ, in japonica-type Korean rice cultivars. The fifth transcript, referred to as OsFCA-e{open}, was similar to OsFCA-γ, except a part of the OsFCA intron 16 was retained. Unlike the FCA-γ protein, the OsFCA-γ protein contains a glycine-rich region at its N-terminus. We detected the OsFCA transcripts missing the region encoding the glycine-rich domain in the indica-type rice, but not in the japonica-type rice. We also found that the OsFCA-δ and OsFCA-e{open} transcripts were expressed in almost all of the different tissue types examined. Taken together, these results indicate that the alternative processing of the OsFCA transcript is more complex than its Arabidopsis counterpart.
| Original language | English |
|---|---|
| Pages (from-to) | 161-166 |
| Number of pages | 6 |
| Journal | Journal of Plant Biology |
| Volume | 52 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 2009 Apr 1 |
Fingerprint
Keywords
- Alternatively spliced transcripts
- Flowering time
- OsFCA
- Rice
ASJC Scopus subject areas
- Plant Science
Cite this
OsFCA transcripts show more complex alternative processing patterns than its Arabidopsis counterparts. / Jang, Yun Hee; Lee, Jeong Hwan; Park, Hyo Young; Kim, Soon Kap; Lee, Bo Young; Suh, Mi Chung; Kim, Jeong Kook.
In: Journal of Plant Biology, Vol. 52, No. 2, 01.04.2009, p. 161-166.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - OsFCA transcripts show more complex alternative processing patterns than its Arabidopsis counterparts
AU - Jang, Yun Hee
AU - Lee, Jeong Hwan
AU - Park, Hyo Young
AU - Kim, Soon Kap
AU - Lee, Bo Young
AU - Suh, Mi Chung
AU - Kim, Jeong Kook
PY - 2009/4/1
Y1 - 2009/4/1
N2 - The FCA gene, which is a component of the autonomous pathway that regulates flowering time, is an important example of how alternative processing can control plant development. We have previously characterized the FCA homolog, OsFCA, from a japonica-type rice cultivar and demonstrated that the polyadenylation site within intron 3, which can generate non-functional FCA-β, was conserved in rice. In this study, we detected five alternatively processed variants of OsFCA pre-mRNA, four of which were equivalents of FCA-α, -β, -γ, and -δ, in japonica-type Korean rice cultivars. The fifth transcript, referred to as OsFCA-e{open}, was similar to OsFCA-γ, except a part of the OsFCA intron 16 was retained. Unlike the FCA-γ protein, the OsFCA-γ protein contains a glycine-rich region at its N-terminus. We detected the OsFCA transcripts missing the region encoding the glycine-rich domain in the indica-type rice, but not in the japonica-type rice. We also found that the OsFCA-δ and OsFCA-e{open} transcripts were expressed in almost all of the different tissue types examined. Taken together, these results indicate that the alternative processing of the OsFCA transcript is more complex than its Arabidopsis counterpart.
AB - The FCA gene, which is a component of the autonomous pathway that regulates flowering time, is an important example of how alternative processing can control plant development. We have previously characterized the FCA homolog, OsFCA, from a japonica-type rice cultivar and demonstrated that the polyadenylation site within intron 3, which can generate non-functional FCA-β, was conserved in rice. In this study, we detected five alternatively processed variants of OsFCA pre-mRNA, four of which were equivalents of FCA-α, -β, -γ, and -δ, in japonica-type Korean rice cultivars. The fifth transcript, referred to as OsFCA-e{open}, was similar to OsFCA-γ, except a part of the OsFCA intron 16 was retained. Unlike the FCA-γ protein, the OsFCA-γ protein contains a glycine-rich region at its N-terminus. We detected the OsFCA transcripts missing the region encoding the glycine-rich domain in the indica-type rice, but not in the japonica-type rice. We also found that the OsFCA-δ and OsFCA-e{open} transcripts were expressed in almost all of the different tissue types examined. Taken together, these results indicate that the alternative processing of the OsFCA transcript is more complex than its Arabidopsis counterpart.
KW - Alternatively spliced transcripts
KW - Flowering time
KW - OsFCA
KW - Rice
UR - http://www.scopus.com/inward/record.url?scp=70349421040&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=70349421040&partnerID=8YFLogxK
U2 - 10.1007/s12374-009-9018-x
DO - 10.1007/s12374-009-9018-x
M3 - Article
AN - SCOPUS:70349421040
VL - 52
SP - 161
EP - 166
JO - Journal of Plant Biology
JF - Journal of Plant Biology
SN - 1226-9239
IS - 2
ER -