TY - GEN
T1 - Osmosensitive gene expression of taurine transporter and cyclin C in embryonic fibroblast cells
AU - Oh, Changkyu
AU - Choi, Yun Jaie
AU - Kim, Hyung Gee
AU - Lee, Dong Hee
PY - 2006/12/1
Y1 - 2006/12/1
N2 - Hypertonic conditions induce osmoregulatory activity. Molecular mechanism, however, remains to be further elucidated concerning the osmosensitive balancing activity. Using a differential display protocol, the genes of taurine transporter (TauT) and cyclin C were identified as candidate responding to the hypertonicity. When a quantitative PCR analysis was performed on the total RNA from KBEF cell treated under hypertonic conditions, the gene expressions of TauT and cyclin C were greatly increased. In terms of protein, the level of TauT expression increased up to 3.2-fold in response to the hypertonic treatment. Similarly to TauT, cyclin C protein also increased 2.4-fold compared to the control treatments. Under taurine-rich extracellular conditions, however, the level of TauT expression increased as little as 1.8-fold by hypertonicity treatment. Cyclin C expression was also lowered compared with low-taurine hypertonic treatment. Cysteine dioxygenase was shown to be highly responsive to the hypertonic treatment. Taken together, these results strongly indicate that the gene expressions of TauT and cyclin C are cooperatively regulated under hypotonic conditions.
AB - Hypertonic conditions induce osmoregulatory activity. Molecular mechanism, however, remains to be further elucidated concerning the osmosensitive balancing activity. Using a differential display protocol, the genes of taurine transporter (TauT) and cyclin C were identified as candidate responding to the hypertonicity. When a quantitative PCR analysis was performed on the total RNA from KBEF cell treated under hypertonic conditions, the gene expressions of TauT and cyclin C were greatly increased. In terms of protein, the level of TauT expression increased up to 3.2-fold in response to the hypertonic treatment. Similarly to TauT, cyclin C protein also increased 2.4-fold compared to the control treatments. Under taurine-rich extracellular conditions, however, the level of TauT expression increased as little as 1.8-fold by hypertonicity treatment. Cyclin C expression was also lowered compared with low-taurine hypertonic treatment. Cysteine dioxygenase was shown to be highly responsive to the hypertonic treatment. Taken together, these results strongly indicate that the gene expressions of TauT and cyclin C are cooperatively regulated under hypotonic conditions.
KW - Cyclin
KW - Cysteine dioxygenase
KW - Hypertonicity
KW - Taurine
UR - http://www.scopus.com/inward/record.url?scp=39049174223&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=39049174223&partnerID=8YFLogxK
U2 - 10.1007/978-0-387-33504-9-5
DO - 10.1007/978-0-387-33504-9-5
M3 - Conference contribution
C2 - 17153588
SN - 9780387323565
VL - 583
T3 - Advances in Experimental Medicine and Biology
SP - 49
EP - 57
BT - Advances in Experimental Medicine and Biology
ER -