Osmosensitive gene expression of taurine transporter and cyclin C in embryonic fibroblast cells

Changkyu Oh, Yun Jaie Choi, Hyung Gee Kim, Dong Hee Lee

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Hypertonic conditions induce osmoregulatory activity. Molecular mechanism, however, remains to be further elucidated concerning the osmosensitive balancing activity. Using a differential display protocol, the genes of taurine transporter (TauT) and cyclin C were identified as candidate responding to the hypertonicity. When a quantitative PCR analysis was performed on the total RNA from KBEF cell treated under hypertonic conditions, the gene expressions of TauT and cyclin C were greatly increased. In terms of protein, the level of TauT expression increased up to 3.2-fold in response to the hypertonic treatment. Similarly to TauT, cyclin C protein also increased 2.4-fold compared to the control treatments. Under taurine-rich extracellular conditions, however, the level of TauT expression increased as little as 1.8-fold by hypertonicity treatment. Cyclin C expression was also lowered compared with low-taurine hypertonic treatment. Cysteine dioxygenase was shown to be highly responsive to the hypertonic treatment. Taken together, these results strongly indicate that the gene expressions of TauT and cyclin C are cooperatively regulated under hypotonic conditions.

Original languageEnglish
Title of host publicationAdvances in Experimental Medicine and Biology
Pages49-57
Number of pages9
Volume583
DOIs
Publication statusPublished - 2006 Dec 1
Externally publishedYes

Publication series

NameAdvances in Experimental Medicine and Biology
Volume583
ISSN (Print)00652598

Fingerprint

Cyclin C
Fibroblasts
Gene expression
Cells
Gene Expression
Taurine
Cysteine Dioxygenase
taurine transporter
Proteins
Genes
Display devices
RNA
Polymerase Chain Reaction

Keywords

  • Cyclin
  • Cysteine dioxygenase
  • Hypertonicity
  • Taurine

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Oh, C., Choi, Y. J., Kim, H. G., & Lee, D. H. (2006). Osmosensitive gene expression of taurine transporter and cyclin C in embryonic fibroblast cells. In Advances in Experimental Medicine and Biology (Vol. 583, pp. 49-57). (Advances in Experimental Medicine and Biology; Vol. 583). https://doi.org/10.1007/978-0-387-33504-9-5

Osmosensitive gene expression of taurine transporter and cyclin C in embryonic fibroblast cells. / Oh, Changkyu; Choi, Yun Jaie; Kim, Hyung Gee; Lee, Dong Hee.

Advances in Experimental Medicine and Biology. Vol. 583 2006. p. 49-57 (Advances in Experimental Medicine and Biology; Vol. 583).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Oh, C, Choi, YJ, Kim, HG & Lee, DH 2006, Osmosensitive gene expression of taurine transporter and cyclin C in embryonic fibroblast cells. in Advances in Experimental Medicine and Biology. vol. 583, Advances in Experimental Medicine and Biology, vol. 583, pp. 49-57. https://doi.org/10.1007/978-0-387-33504-9-5
Oh C, Choi YJ, Kim HG, Lee DH. Osmosensitive gene expression of taurine transporter and cyclin C in embryonic fibroblast cells. In Advances in Experimental Medicine and Biology. Vol. 583. 2006. p. 49-57. (Advances in Experimental Medicine and Biology). https://doi.org/10.1007/978-0-387-33504-9-5
Oh, Changkyu ; Choi, Yun Jaie ; Kim, Hyung Gee ; Lee, Dong Hee. / Osmosensitive gene expression of taurine transporter and cyclin C in embryonic fibroblast cells. Advances in Experimental Medicine and Biology. Vol. 583 2006. pp. 49-57 (Advances in Experimental Medicine and Biology).
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