OsMPK3 is a TEY-type rice MAPK in Group C and phosphorylates OsbHLH65, a transcription factor binding to the E-box element

Hyun Young Shin, Min Kyoung You, Ji Ung Jeung, Jeong Sheop Shin

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Key message: OsMPK3 is a TEY-type rice MAPK belonging to Group C and directly phosphorylates OsbHLH65 in the nucleus. OsMPK3 and OsbHLH65 are induced by biotic stress and defense-related hormones. Mitogen-activated protein kinases (MAPKs) are involved in the majority of signaling pathways that regulate plant development and stress tolerance via the phosphorylation of target molecules. Plant MAPKs are classified into two subtypes, TEY and TDY, according to the TxY (x = E or D) motif in their activation loop, and the TDY motif is unique to plant MAPKs. In rice, 17 MAPKs have been classified into six groups. To date, the functions of many TDY-type rice MAPKs have been characterized, but little is known of the TEY-type MAPKs in Group C and their possible target substrates. In the study reported here, we determined that a TEY-type rice MAPK belonging to subgroup C, named OsMPK3, phosphorylates its substrate OsbHLH65 in the nucleus. Our electrophoresis mobility shift assay results revealed that OsbHLH65 specifically binds to the E-box cis-element, but not to the G-box. Both OsMPK3 and OsbHLH65 were induced by treatments with rice blast (Magnaporthe grisea), brown planthopper (Nilaparvata lugens), and defense-related hormones, such as methyl jasmonic acid and salicylic acid. Our results suggest the possibility that OsMPK3 contributes to the defense signal transduction by phosphorylating the basic helix-loop-helix transcription factor.

Original languageEnglish
Pages (from-to)1343-1353
Number of pages11
JournalPlant Cell Reports
Volume33
Issue number8
DOIs
Publication statusPublished - 2014 Jan 1

Fingerprint

mitogen-activated protein kinase
transcription factors
rice
Nilaparvata lugens
hormones
basic helix-loop-helix transcription factors
Magnaporthe grisea
blast disease
plant stress
methyl jasmonate
biotic stress
salicylic acid
stress tolerance
electrophoresis
plant development
signal transduction
phosphorylation
assays

ASJC Scopus subject areas

  • Plant Science
  • Agronomy and Crop Science

Cite this

OsMPK3 is a TEY-type rice MAPK in Group C and phosphorylates OsbHLH65, a transcription factor binding to the E-box element. / Shin, Hyun Young; You, Min Kyoung; Jeung, Ji Ung; Shin, Jeong Sheop.

In: Plant Cell Reports, Vol. 33, No. 8, 01.01.2014, p. 1343-1353.

Research output: Contribution to journalArticle

@article{5001c9246f7e44f68ab7ad71d40d2c84,
title = "OsMPK3 is a TEY-type rice MAPK in Group C and phosphorylates OsbHLH65, a transcription factor binding to the E-box element",
abstract = "Key message: OsMPK3 is a TEY-type rice MAPK belonging to Group C and directly phosphorylates OsbHLH65 in the nucleus. OsMPK3 and OsbHLH65 are induced by biotic stress and defense-related hormones. Mitogen-activated protein kinases (MAPKs) are involved in the majority of signaling pathways that regulate plant development and stress tolerance via the phosphorylation of target molecules. Plant MAPKs are classified into two subtypes, TEY and TDY, according to the TxY (x = E or D) motif in their activation loop, and the TDY motif is unique to plant MAPKs. In rice, 17 MAPKs have been classified into six groups. To date, the functions of many TDY-type rice MAPKs have been characterized, but little is known of the TEY-type MAPKs in Group C and their possible target substrates. In the study reported here, we determined that a TEY-type rice MAPK belonging to subgroup C, named OsMPK3, phosphorylates its substrate OsbHLH65 in the nucleus. Our electrophoresis mobility shift assay results revealed that OsbHLH65 specifically binds to the E-box cis-element, but not to the G-box. Both OsMPK3 and OsbHLH65 were induced by treatments with rice blast (Magnaporthe grisea), brown planthopper (Nilaparvata lugens), and defense-related hormones, such as methyl jasmonic acid and salicylic acid. Our results suggest the possibility that OsMPK3 contributes to the defense signal transduction by phosphorylating the basic helix-loop-helix transcription factor.",
keywords = "Basic helix-loop-helix, Biotic stress, E-box cis-element, Magnaporthe grisea, MAP kinase, Oryza sativa",
author = "Shin, {Hyun Young} and You, {Min Kyoung} and Jeung, {Ji Ung} and Shin, {Jeong Sheop}",
year = "2014",
month = "1",
day = "1",
doi = "10.1007/s00299-014-1620-9",
language = "English",
volume = "33",
pages = "1343--1353",
journal = "Plant Cell Reports",
issn = "0721-7714",
publisher = "Springer Verlag",
number = "8",

}

TY - JOUR

T1 - OsMPK3 is a TEY-type rice MAPK in Group C and phosphorylates OsbHLH65, a transcription factor binding to the E-box element

AU - Shin, Hyun Young

AU - You, Min Kyoung

AU - Jeung, Ji Ung

AU - Shin, Jeong Sheop

PY - 2014/1/1

Y1 - 2014/1/1

N2 - Key message: OsMPK3 is a TEY-type rice MAPK belonging to Group C and directly phosphorylates OsbHLH65 in the nucleus. OsMPK3 and OsbHLH65 are induced by biotic stress and defense-related hormones. Mitogen-activated protein kinases (MAPKs) are involved in the majority of signaling pathways that regulate plant development and stress tolerance via the phosphorylation of target molecules. Plant MAPKs are classified into two subtypes, TEY and TDY, according to the TxY (x = E or D) motif in their activation loop, and the TDY motif is unique to plant MAPKs. In rice, 17 MAPKs have been classified into six groups. To date, the functions of many TDY-type rice MAPKs have been characterized, but little is known of the TEY-type MAPKs in Group C and their possible target substrates. In the study reported here, we determined that a TEY-type rice MAPK belonging to subgroup C, named OsMPK3, phosphorylates its substrate OsbHLH65 in the nucleus. Our electrophoresis mobility shift assay results revealed that OsbHLH65 specifically binds to the E-box cis-element, but not to the G-box. Both OsMPK3 and OsbHLH65 were induced by treatments with rice blast (Magnaporthe grisea), brown planthopper (Nilaparvata lugens), and defense-related hormones, such as methyl jasmonic acid and salicylic acid. Our results suggest the possibility that OsMPK3 contributes to the defense signal transduction by phosphorylating the basic helix-loop-helix transcription factor.

AB - Key message: OsMPK3 is a TEY-type rice MAPK belonging to Group C and directly phosphorylates OsbHLH65 in the nucleus. OsMPK3 and OsbHLH65 are induced by biotic stress and defense-related hormones. Mitogen-activated protein kinases (MAPKs) are involved in the majority of signaling pathways that regulate plant development and stress tolerance via the phosphorylation of target molecules. Plant MAPKs are classified into two subtypes, TEY and TDY, according to the TxY (x = E or D) motif in their activation loop, and the TDY motif is unique to plant MAPKs. In rice, 17 MAPKs have been classified into six groups. To date, the functions of many TDY-type rice MAPKs have been characterized, but little is known of the TEY-type MAPKs in Group C and their possible target substrates. In the study reported here, we determined that a TEY-type rice MAPK belonging to subgroup C, named OsMPK3, phosphorylates its substrate OsbHLH65 in the nucleus. Our electrophoresis mobility shift assay results revealed that OsbHLH65 specifically binds to the E-box cis-element, but not to the G-box. Both OsMPK3 and OsbHLH65 were induced by treatments with rice blast (Magnaporthe grisea), brown planthopper (Nilaparvata lugens), and defense-related hormones, such as methyl jasmonic acid and salicylic acid. Our results suggest the possibility that OsMPK3 contributes to the defense signal transduction by phosphorylating the basic helix-loop-helix transcription factor.

KW - Basic helix-loop-helix

KW - Biotic stress

KW - E-box cis-element

KW - Magnaporthe grisea

KW - MAP kinase

KW - Oryza sativa

UR - http://www.scopus.com/inward/record.url?scp=84904480060&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84904480060&partnerID=8YFLogxK

U2 - 10.1007/s00299-014-1620-9

DO - 10.1007/s00299-014-1620-9

M3 - Article

C2 - 24777821

AN - SCOPUS:84904480060

VL - 33

SP - 1343

EP - 1353

JO - Plant Cell Reports

JF - Plant Cell Reports

SN - 0721-7714

IS - 8

ER -