OsNF-YC2 and OsNF-YC4 proteins inhibit flowering under long-day conditions in rice

Soon Kap Kim, Hyo Young Park, Yun Hee Jang, Keh Chien Lee, Young Soo Chung, Jeong Hwan Lee, Jeong Kook Kim

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Main conclusion: OsNF-YC2 and OsNF-YC4 proteins regulate the photoperiodic flowering response through the modulation of three flowering-time genes (Ehd1, Hd3a, andRFT1) in rice. Plant NUCLEAR FACTOR Y (NF-Y) transcription factors control numerous developmental processes by forming heterotrimeric complexes, but little is known about their roles in flowering in rice. In this study, it is shown that some subunits of OsNF-YB and OsNF-YC interact with each other, and among them, OsNF-YC2 and OsNF-YC4 proteins regulate the photoperiodic flowering response of rice. Protein interaction studies showed that the physical interactions occurred between the three OsNF-YC proteins (OsNF-YC2, OsNF-YC4 and OsNF-YC6) and three OsNF-YB proteins (OsNF-YB8, OsNF-YB10 and OsNF-YB11). Repression and overexpression of the OsNF-YC2 and OsNF-YC4 genes revealed that they act as inhibitors of flowering only under long-day (LD) conditions. Overexpression of OsNF-YC6, however, promoted flowering only under LD conditions, suggesting it could function as a flowering promoter. These phenotypes correlated with the changes in the expression of three rice flowering-time genes [Early heading date 1 (Ehd1), Heading date 3a (Hd3a) and RICE FLOWERING LOCUS T1 (RFT1)]. The diurnal and tissue-specific expression patterns of the subsets of OsNF-YB and OsNF-YC genes were similar to those of CCT domain encoding genes such as OsCO3, Heading date 1 (Hd1) and Ghd7. We propose that OsNF-YC2 and OsNF-YC4 proteins regulate the photoperiodic flowering response by interacting directly with OsNF-YB8, OsNF-YB10 or OsNF-YB11 proteins in rice.

Original languageEnglish
Pages (from-to)563-576
Number of pages14
JournalPlanta
Volume243
Issue number3
DOIs
Publication statusPublished - 2016 Mar 1

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flowering
rice
heading
Proteins
proteins
Genes
genes
Oryza
rice protein
Transcription Factors
Phenotype
transcription factors
promoter regions
phenotype
loci

Keywords

  • Flowering time
  • NUCLEAR FACTOR Y (NF-Y)
  • Photoperiodic flowering
  • Rice
  • Transcription

ASJC Scopus subject areas

  • Plant Science
  • Genetics

Cite this

Kim, S. K., Park, H. Y., Jang, Y. H., Lee, K. C., Chung, Y. S., Lee, J. H., & Kim, J. K. (2016). OsNF-YC2 and OsNF-YC4 proteins inhibit flowering under long-day conditions in rice. Planta, 243(3), 563-576. https://doi.org/10.1007/s00425-015-2426-x

OsNF-YC2 and OsNF-YC4 proteins inhibit flowering under long-day conditions in rice. / Kim, Soon Kap; Park, Hyo Young; Jang, Yun Hee; Lee, Keh Chien; Chung, Young Soo; Lee, Jeong Hwan; Kim, Jeong Kook.

In: Planta, Vol. 243, No. 3, 01.03.2016, p. 563-576.

Research output: Contribution to journalArticle

Kim, SK, Park, HY, Jang, YH, Lee, KC, Chung, YS, Lee, JH & Kim, JK 2016, 'OsNF-YC2 and OsNF-YC4 proteins inhibit flowering under long-day conditions in rice', Planta, vol. 243, no. 3, pp. 563-576. https://doi.org/10.1007/s00425-015-2426-x
Kim SK, Park HY, Jang YH, Lee KC, Chung YS, Lee JH et al. OsNF-YC2 and OsNF-YC4 proteins inhibit flowering under long-day conditions in rice. Planta. 2016 Mar 1;243(3):563-576. https://doi.org/10.1007/s00425-015-2426-x
Kim, Soon Kap ; Park, Hyo Young ; Jang, Yun Hee ; Lee, Keh Chien ; Chung, Young Soo ; Lee, Jeong Hwan ; Kim, Jeong Kook. / OsNF-YC2 and OsNF-YC4 proteins inhibit flowering under long-day conditions in rice. In: Planta. 2016 ; Vol. 243, No. 3. pp. 563-576.
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abstract = "Main conclusion: OsNF-YC2 and OsNF-YC4 proteins regulate the photoperiodic flowering response through the modulation of three flowering-time genes (Ehd1, Hd3a, andRFT1) in rice. Plant NUCLEAR FACTOR Y (NF-Y) transcription factors control numerous developmental processes by forming heterotrimeric complexes, but little is known about their roles in flowering in rice. In this study, it is shown that some subunits of OsNF-YB and OsNF-YC interact with each other, and among them, OsNF-YC2 and OsNF-YC4 proteins regulate the photoperiodic flowering response of rice. Protein interaction studies showed that the physical interactions occurred between the three OsNF-YC proteins (OsNF-YC2, OsNF-YC4 and OsNF-YC6) and three OsNF-YB proteins (OsNF-YB8, OsNF-YB10 and OsNF-YB11). Repression and overexpression of the OsNF-YC2 and OsNF-YC4 genes revealed that they act as inhibitors of flowering only under long-day (LD) conditions. Overexpression of OsNF-YC6, however, promoted flowering only under LD conditions, suggesting it could function as a flowering promoter. These phenotypes correlated with the changes in the expression of three rice flowering-time genes [Early heading date 1 (Ehd1), Heading date 3a (Hd3a) and RICE FLOWERING LOCUS T1 (RFT1)]. The diurnal and tissue-specific expression patterns of the subsets of OsNF-YB and OsNF-YC genes were similar to those of CCT domain encoding genes such as OsCO3, Heading date 1 (Hd1) and Ghd7. We propose that OsNF-YC2 and OsNF-YC4 proteins regulate the photoperiodic flowering response by interacting directly with OsNF-YB8, OsNF-YB10 or OsNF-YB11 proteins in rice.",
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AU - Chung, Young Soo

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