OsPRP3, a flower specific Proline-rich protein of rice, determines extracellular matrix structure of floral organs and its overexpression confers cold-tolerance

Kodiveri Muthukalianan Gothandam, Easwaran Nalini, Sivashanmugam Karthikeyan, Jeong Sheop Shin

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Proline-rich protein (PRP), a cell wall protein of plant, has been studied in many plant species. Yet, none of the PRPs has been functionally elucidated. Here we report a novel flower-specific PRP designated OsPRP3 from rice. Expression analysis showed that the OsPRP3 transcript was mainly present in rice flower and accumulated abundantly during the late stage of the flower development. To study the function of OsPRP3, we constructed and transformed a binary vector containing a full clone of OsPRP3 in sense orientation and also an RNAi vector to achieve overexpression and knockout of the gene, respectively. Our overexpression plants showed a significant increase in cold tolerance than the WT plants which is conferred by the accumulation of OsPRP3 protein during cold treatment. Further the microscopic analysis revealed that OsPRP3 enhances the cell wall integrity in the cold tolerant plant and confers cold-tolerance in rice. Microscopic analysis of the RNAi mutant flower revealed that blocking OsPRP3 function caused significant defects in floral organogenesis. Taken together, the results suggested that OsPRP3 is a cell wall protein, playing a crucial role in determining extracellular matrix structure of floral organs.

Original languageEnglish
Pages (from-to)125-135
Number of pages11
JournalPlant Molecular Biology
Volume72
Issue number1-2
DOIs
Publication statusPublished - 2010 Jan 1

Fingerprint

rice protein
extracellular matrix
Proline
cold tolerance
Extracellular Matrix
proline
Cell Wall
flowers
cell walls
RNA Interference
rice
Proteins
proteins
Plant Proteins
Gene Knockout Techniques
gene overexpression
Organogenesis
gene targeting
plant proteins
organogenesis

Keywords

  • Cold tolerance
  • Flower development
  • Proline rich protein
  • Rice

ASJC Scopus subject areas

  • Plant Science
  • Agronomy and Crop Science
  • Genetics

Cite this

OsPRP3, a flower specific Proline-rich protein of rice, determines extracellular matrix structure of floral organs and its overexpression confers cold-tolerance. / Gothandam, Kodiveri Muthukalianan; Nalini, Easwaran; Karthikeyan, Sivashanmugam; Shin, Jeong Sheop.

In: Plant Molecular Biology, Vol. 72, No. 1-2, 01.01.2010, p. 125-135.

Research output: Contribution to journalArticle

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