Osteoblast activity of MG-63 cells is enhanced by growth on a lactoferrin-immobilized titanium substrate

Sung Eun Kim, Young Pil Yun, Jae Yong Lee, Kyeongsoon Park, Dong Hun Suh

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The aim of this study was to develop a lactoferrin (LF)-immobilized titanium (Ti) substrate to enhance the osteoblast activity of MG-63 cells. Ti substrates were first modified through heparin-dopamine (Hep-DOPA) anchorage. Then, LF was immobilized on the Hep-Ti substrates via electrostatic interactions. Hep-Ti substrates, with or without LF, were evaluated by scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS), and contact angle measurements. Sustained release of LF on the Ti substrates was observed over a 28-day period. In vitro studies of osteoblast activity showed increased alkaline phosphatase activity and calcium deposition by MG-63 cells cultured on LF-immobilized Ti substrates as compared to those cultured on pristine Ti substrates, indicating that LF-immobilized Ti substrates were effective at enhancing osteoblast activity.

Original languageEnglish
Pages (from-to)191-198
Number of pages8
JournalColloids and Surfaces B: Biointerfaces
Volume123
DOIs
Publication statusPublished - 2014 Nov 1

Fingerprint

osteoblasts
Lactoferrin
Osteoblasts
Titanium
titanium
Substrates
Growth
cells
heparins
Photoelectron Spectroscopy
dopamine
phosphatases
Phosphatases
Angle measurement
Coulomb interactions
Static Electricity
cultured cells
Electron Scanning Microscopy
Contact angle
Alkaline Phosphatase

Keywords

  • Heparin-dopamine
  • Lactoferrin
  • Osteoblast
  • Osteogenic differentiation
  • Titanium

ASJC Scopus subject areas

  • Biotechnology
  • Surfaces and Interfaces
  • Physical and Theoretical Chemistry
  • Colloid and Surface Chemistry

Cite this

Osteoblast activity of MG-63 cells is enhanced by growth on a lactoferrin-immobilized titanium substrate. / Kim, Sung Eun; Yun, Young Pil; Lee, Jae Yong; Park, Kyeongsoon; Suh, Dong Hun.

In: Colloids and Surfaces B: Biointerfaces, Vol. 123, 01.11.2014, p. 191-198.

Research output: Contribution to journalArticle

Kim, Sung Eun ; Yun, Young Pil ; Lee, Jae Yong ; Park, Kyeongsoon ; Suh, Dong Hun. / Osteoblast activity of MG-63 cells is enhanced by growth on a lactoferrin-immobilized titanium substrate. In: Colloids and Surfaces B: Biointerfaces. 2014 ; Vol. 123. pp. 191-198.
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