Osteoblast activity of MG-63 cells is enhanced by growth on a lactoferrin-immobilized titanium substrate

Sung Eun Kim, Young Pil Yun, Jae Yong Lee, Kyeongsoon Park, Dong Hun Suh

    Research output: Contribution to journalArticlepeer-review

    16 Citations (Scopus)

    Abstract

    The aim of this study was to develop a lactoferrin (LF)-immobilized titanium (Ti) substrate to enhance the osteoblast activity of MG-63 cells. Ti substrates were first modified through heparin-dopamine (Hep-DOPA) anchorage. Then, LF was immobilized on the Hep-Ti substrates via electrostatic interactions. Hep-Ti substrates, with or without LF, were evaluated by scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS), and contact angle measurements. Sustained release of LF on the Ti substrates was observed over a 28-day period. In vitro studies of osteoblast activity showed increased alkaline phosphatase activity and calcium deposition by MG-63 cells cultured on LF-immobilized Ti substrates as compared to those cultured on pristine Ti substrates, indicating that LF-immobilized Ti substrates were effective at enhancing osteoblast activity.

    Original languageEnglish
    Pages (from-to)191-198
    Number of pages8
    JournalColloids and Surfaces B: Biointerfaces
    Volume123
    DOIs
    Publication statusPublished - 2014 Nov 1

    Keywords

    • Heparin-dopamine
    • Lactoferrin
    • Osteoblast
    • Osteogenic differentiation
    • Titanium

    ASJC Scopus subject areas

    • Biotechnology
    • Surfaces and Interfaces
    • Physical and Theoretical Chemistry
    • Colloid and Surface Chemistry

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