Osteoconductivity of titanium implants coated with a new fusion protein containing four cell adhesion motifs

Jin Woo Park, Dong Hee Lee, Youn Jeong Kim, Je Hee Jang, Jo Young Suh, In San Kim

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

This study investigated the effects of a new recombinant protein(T-CAM) coating comprising four cell adhesion motifs for integrins(Arg-Gly-Asp and Pro-His-Ser-Arg-Asn motifs from fibronectin, and Glu-Pro-Asp-Ile- Met and Try-His motifs from βig-h3) on the osteoconductivity of titanium implant surfaces in vitro and in vivo. Osteoblast cell response including cell adhesion, proliferation, and alkaline phosphatase(ALP) activity to uncoated and T-CAM coated machined titanium surfaces were investigated using MG63 osteoblast-like cells. The osteoconductivity of T-CAM coated machined titanium implants was evaluated byremoval torque testing and histomorphometric analysis after 6 weeks of implantation in rabbit tibiae. T-CAM coated surfaces showed significantly increased osteoblastic cell adhesion, proliferation, ALP activity, removal torque forces, and bone-implant contact values compared with those of uncoated surfaces(P < 0.05). These results indicate that a simple coating of T-CAM, comprising four cell adhesion motifs, may enhance bone integration of titanium implants by promoting ostoblast cell adhesion, proliferation and differentiation on their surfaces.

Original languageEnglish
Pages (from-to)888-892
Number of pages5
JournalTissue Engineering and Regenerative Medicine
Volume6
Issue number4-11
Publication statusPublished - 2009 Jun
Externally publishedYes

Keywords

  • Cell adhesion molecule
  • Histomorphometry
  • Osseointegration
  • Osteoblast
  • Titanium implant

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Biomedical Engineering

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