Osteogenic potential of non thermal biocompatible atmospheric pressure plasma treated zirconia: In vitro study

Nayansi Jha, Jin Sung Choi, Ji Hye Kim, Ranju Jung, Eun Ha Choi, Jae Jun Ryu, Ihn Han

    Research output: Contribution to journalArticlepeer-review

    7 Citations (Scopus)

    Abstract

    Zirconia has recently gained importance as a dental implant material due to its favourable properties. The present study aimed to evaluate the osteogenic potential of Non Thermal Atmospheric Pressure Plasma treated YTZP using O2 and N2 gases. The proliferation and differentiation of osteoblastic cell-line MC3T3-E1 was tested using dielectric barrier discharge plasma as a new technique. YTZP discs were divided into control (untreated) and plasma (O2 or N2) treated groups on which MC3T3-E1 osteoblastic cells were seeded. The specimens were tested for cell cytotoxicity and differentiation and surface characteristics were analysed using scanning electron microscope (SEM), X-ray photoelectron spectroscopy (XPS), Energy dispersive X-ray spectroscopy (EDS) and contact angle analysis. Surface analysis indicated generation of zirconium oxide nanoparticles on the discs and the contact angle decreased slightly on treatment with O2 and N2 gas. XPS and EDS analysis revealed the structural effects on YTZP surface due to plasma treatment. Alizarin Red Staining indicated increased osteogenic response on plasma coated YTZP discs (N2Effect > O2Effect). The overall effect of plasma surface treatment was positive for MC3T3-E1 osteoblasts. The results suggest that plasma treatment improves the YTZP surface and is favourable for osteogenesis.

    Original languageEnglish
    Pages (from-to)662-670
    Number of pages9
    JournalJournal of Biomaterials and Tissue Engineering
    Volume7
    Issue number8
    DOIs
    Publication statusPublished - 2017

    Keywords

    • Dental implants
    • Dielectric barrier discharge (DBD)
    • MC3T3-E1 osteoblasts
    • Non-thermal biocompatible atmospheric plasma (NBP)
    • Osseointegration
    • YTZP (yttria stabilized tetragonal zirconia)

    ASJC Scopus subject areas

    • Biotechnology
    • Bioengineering
    • Medicine (miscellaneous)
    • Biomedical Engineering

    Fingerprint

    Dive into the research topics of 'Osteogenic potential of non thermal biocompatible atmospheric pressure plasma treated zirconia: In vitro study'. Together they form a unique fingerprint.

    Cite this