Abstract
Zirconia has recently gained importance as a dental implant material due to its favourable properties. The present study aimed to evaluate the osteogenic potential of Non Thermal Atmospheric Pressure Plasma treated YTZP using O2 and N2 gases. The proliferation and differentiation of osteoblastic cell-line MC3T3-E1 was tested using dielectric barrier discharge plasma as a new technique. YTZP discs were divided into control (untreated) and plasma (O2 or N2) treated groups on which MC3T3-E1 osteoblastic cells were seeded. The specimens were tested for cell cytotoxicity and differentiation and surface characteristics were analysed using scanning electron microscope (SEM), X-ray photoelectron spectroscopy (XPS), Energy dispersive X-ray spectroscopy (EDS) and contact angle analysis. Surface analysis indicated generation of zirconium oxide nanoparticles on the discs and the contact angle decreased slightly on treatment with O2 and N2 gas. XPS and EDS analysis revealed the structural effects on YTZP surface due to plasma treatment. Alizarin Red Staining indicated increased osteogenic response on plasma coated YTZP discs (N2Effect > O2Effect). The overall effect of plasma surface treatment was positive for MC3T3-E1 osteoblasts. The results suggest that plasma treatment improves the YTZP surface and is favourable for osteogenesis.
| Original language | English |
|---|---|
| Pages (from-to) | 662-670 |
| Number of pages | 9 |
| Journal | Journal of Biomaterials and Tissue Engineering |
| Volume | 7 |
| Issue number | 8 |
| DOIs | |
| Publication status | Published - 2017 Jan 1 |
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Keywords
- Dental implants
- Dielectric barrier discharge (DBD)
- MC3T3-E1 osteoblasts
- Non-thermal biocompatible atmospheric plasma (NBP)
- Osseointegration
- YTZP (yttria stabilized tetragonal zirconia)
ASJC Scopus subject areas
- Biotechnology
- Bioengineering
- Medicine (miscellaneous)
- Biomedical Engineering
Cite this
Osteogenic potential of non thermal biocompatible atmospheric pressure plasma treated zirconia : In vitro study. / Jha, Nayansi; Choi, Jin Sung; Kim, Ji Hye; Jung, Ranju; Choi, Eun Ha; Ryu, Jae Jun; Han, Ihn.
In: Journal of Biomaterials and Tissue Engineering, Vol. 7, No. 8, 01.01.2017, p. 662-670.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Osteogenic potential of non thermal biocompatible atmospheric pressure plasma treated zirconia
T2 - In vitro study
AU - Jha, Nayansi
AU - Choi, Jin Sung
AU - Kim, Ji Hye
AU - Jung, Ranju
AU - Choi, Eun Ha
AU - Ryu, Jae Jun
AU - Han, Ihn
PY - 2017/1/1
Y1 - 2017/1/1
N2 - Zirconia has recently gained importance as a dental implant material due to its favourable properties. The present study aimed to evaluate the osteogenic potential of Non Thermal Atmospheric Pressure Plasma treated YTZP using O2 and N2 gases. The proliferation and differentiation of osteoblastic cell-line MC3T3-E1 was tested using dielectric barrier discharge plasma as a new technique. YTZP discs were divided into control (untreated) and plasma (O2 or N2) treated groups on which MC3T3-E1 osteoblastic cells were seeded. The specimens were tested for cell cytotoxicity and differentiation and surface characteristics were analysed using scanning electron microscope (SEM), X-ray photoelectron spectroscopy (XPS), Energy dispersive X-ray spectroscopy (EDS) and contact angle analysis. Surface analysis indicated generation of zirconium oxide nanoparticles on the discs and the contact angle decreased slightly on treatment with O2 and N2 gas. XPS and EDS analysis revealed the structural effects on YTZP surface due to plasma treatment. Alizarin Red Staining indicated increased osteogenic response on plasma coated YTZP discs (N2Effect > O2Effect). The overall effect of plasma surface treatment was positive for MC3T3-E1 osteoblasts. The results suggest that plasma treatment improves the YTZP surface and is favourable for osteogenesis.
AB - Zirconia has recently gained importance as a dental implant material due to its favourable properties. The present study aimed to evaluate the osteogenic potential of Non Thermal Atmospheric Pressure Plasma treated YTZP using O2 and N2 gases. The proliferation and differentiation of osteoblastic cell-line MC3T3-E1 was tested using dielectric barrier discharge plasma as a new technique. YTZP discs were divided into control (untreated) and plasma (O2 or N2) treated groups on which MC3T3-E1 osteoblastic cells were seeded. The specimens were tested for cell cytotoxicity and differentiation and surface characteristics were analysed using scanning electron microscope (SEM), X-ray photoelectron spectroscopy (XPS), Energy dispersive X-ray spectroscopy (EDS) and contact angle analysis. Surface analysis indicated generation of zirconium oxide nanoparticles on the discs and the contact angle decreased slightly on treatment with O2 and N2 gas. XPS and EDS analysis revealed the structural effects on YTZP surface due to plasma treatment. Alizarin Red Staining indicated increased osteogenic response on plasma coated YTZP discs (N2Effect > O2Effect). The overall effect of plasma surface treatment was positive for MC3T3-E1 osteoblasts. The results suggest that plasma treatment improves the YTZP surface and is favourable for osteogenesis.
KW - Dental implants
KW - Dielectric barrier discharge (DBD)
KW - MC3T3-E1 osteoblasts
KW - Non-thermal biocompatible atmospheric plasma (NBP)
KW - Osseointegration
KW - YTZP (yttria stabilized tetragonal zirconia)
UR - http://www.scopus.com/inward/record.url?scp=85027579703&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85027579703&partnerID=8YFLogxK
U2 - 10.1166/jbt.2017.1626
DO - 10.1166/jbt.2017.1626
M3 - Article
AN - SCOPUS:85027579703
VL - 7
SP - 662
EP - 670
JO - Journal of Biomaterials and Tissue Engineering
JF - Journal of Biomaterials and Tissue Engineering
SN - 2157-9083
IS - 8
ER -