O2 plasma patterning of p-type MWCNT and its application to immunosensor

Jun Yong Lee, Eun Jin Park, Joon Hyub Kim, Sun Guk Kim, Cheol Jin Lee, Ja Kim Min, Nam Ki Min

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3 Citations (Scopus)

Abstract

An electrochemical immunosensor based on patterned plasma-functionalized multiwalled carbon nanotubes (pf-MWCNTs) working electrode for Legionella pneumophila detection is described in this paper. Polyclonal antibody was immobilized using passive adsorption and covalent immobilization on the surface of the pf-MWCNTs. Various dilutions of Legionella 19-kDa peptidoglycan- associated lipoprotein (PAL) were added onto the electrode surface and incubated. The assay was then completed using polyclonal antibody conjugated to horseradish peroxidase (HRP) as the enzyme label. 3,3′,5,5′- Tetramethylbenzidine dihydrochloride (TMB)/substrate solution (H 2O2) was used as the mediator/substrate system. Electrochemical detection was conducted using cyclic voltammetric at 500 mV versus silver/silver chloride (Ag/AgCl) reference electrode. L. pneumophila detection of 0.16 ng ml- 1 and ∼ 0.0025 ng ml- 1 was achieved respectively for passive adsorption and covalent immobilization.

Original languageEnglish
Pages (from-to)6646-6649
Number of pages4
JournalThin Solid Films
Volume518
Issue number22
DOIs
Publication statusPublished - 2010 Sep 1

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Keywords

  • ELISA
  • Immunosensors
  • Legionella pneumophila
  • MWCNTs
  • Plasma-pattern

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Materials Chemistry
  • Metals and Alloys
  • Surfaces, Coatings and Films
  • Surfaces and Interfaces

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