Over-expression of neuropeptide urocortin and its receptors in human allergic nasal mucosa

Tae-Hoon Kim, Sang Hag Lee, Heung Man Lee, Seung Hoon Lee, Se Woo Lee, Woo Joo Kim, Se Jin Park, Yang Soo Kim, Hwan Choe, Ho Yeon Hwang, Ik One Yoo

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

OBJECTIVES: Urocortin (UCN) is a member of the corticotropin releasing factor (CRF) neuropeptide family. UCN act as locally expressed proinflammatory factor and induce mast cell degranulation, cytokine secretion, and trigger vascular permeability, which are mediated by CRF receptors in peripheral tissues. Considering its functional roles, UCN and its receptors may play a role in the pathogenesis of allergic nasal mucosa. Therefore, we investigated the expression profile and distribution of UCN and CRF receptors in normal and allergic nasal mucosa. METHODS: Reverse transcriptase-polymerase chain reaction, immunohistochemistry, and Western blotting were applied to the normal and allergic nasal mucosa. RESULTS: The expression levels of UCN and CRF receptors were increased in allergic nasal mucosa in comparison with normal nasal mucosa. In normal nasal mucosa, UCN and CRF receptors were restricted to the vascular endothelium of submucosal cavernous sinusoids where faint staining was found. However, in allergic nasal mucosa, UCN was expressed in small vessels distributed in lamina propria and the vascular endothelium of cavernous sinusoid located in submucosa. Many scattered positive cells were also found in allergic nasal mucosa, probably UCN-positive leukocytes. CRF receptors were also localized in the vascular endothelium of small vessels and cavernous sinusoid. CONCLUSIONS: These results indicate that UCN may play a role in the regulation of vascular swelling in normal nasal mucosa. Moreover, in allergic nasal mucosa, increased expression levels of UCN and its receptors may contribute to increased mucosal swelling and vascular permeability, playing an important role in the pathogenesis of allergic rhinitis.

Original languageEnglish
Pages (from-to)1513-1518
Number of pages6
JournalLaryngoscope
Volume117
Issue number9
DOIs
Publication statusPublished - 2007 Sep 1

Fingerprint

Urocortins
Nasal Mucosa
Neuropeptides
Corticotropin-Releasing Hormone Receptors
Vascular Endothelium
Capillary Permeability
Cell Degranulation
Corticotropin-Releasing Hormone
Reverse Transcriptase Polymerase Chain Reaction
Mast Cells
Blood Vessels
Mucous Membrane
Leukocytes
Western Blotting
Immunohistochemistry

Keywords

  • Allergic nasal mucosa
  • Corticotrophin releasing factor receptors
  • Urocortin

ASJC Scopus subject areas

  • Otorhinolaryngology

Cite this

Over-expression of neuropeptide urocortin and its receptors in human allergic nasal mucosa. / Kim, Tae-Hoon; Lee, Sang Hag; Lee, Heung Man; Lee, Seung Hoon; Lee, Se Woo; Kim, Woo Joo; Park, Se Jin; Kim, Yang Soo; Choe, Hwan; Hwang, Ho Yeon; Yoo, Ik One.

In: Laryngoscope, Vol. 117, No. 9, 01.09.2007, p. 1513-1518.

Research output: Contribution to journalArticle

Kim, Tae-Hoon ; Lee, Sang Hag ; Lee, Heung Man ; Lee, Seung Hoon ; Lee, Se Woo ; Kim, Woo Joo ; Park, Se Jin ; Kim, Yang Soo ; Choe, Hwan ; Hwang, Ho Yeon ; Yoo, Ik One. / Over-expression of neuropeptide urocortin and its receptors in human allergic nasal mucosa. In: Laryngoscope. 2007 ; Vol. 117, No. 9. pp. 1513-1518.
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AU - Lee, Sang Hag

AU - Lee, Heung Man

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AU - Lee, Se Woo

AU - Kim, Woo Joo

AU - Park, Se Jin

AU - Kim, Yang Soo

AU - Choe, Hwan

AU - Hwang, Ho Yeon

AU - Yoo, Ik One

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N2 - OBJECTIVES: Urocortin (UCN) is a member of the corticotropin releasing factor (CRF) neuropeptide family. UCN act as locally expressed proinflammatory factor and induce mast cell degranulation, cytokine secretion, and trigger vascular permeability, which are mediated by CRF receptors in peripheral tissues. Considering its functional roles, UCN and its receptors may play a role in the pathogenesis of allergic nasal mucosa. Therefore, we investigated the expression profile and distribution of UCN and CRF receptors in normal and allergic nasal mucosa. METHODS: Reverse transcriptase-polymerase chain reaction, immunohistochemistry, and Western blotting were applied to the normal and allergic nasal mucosa. RESULTS: The expression levels of UCN and CRF receptors were increased in allergic nasal mucosa in comparison with normal nasal mucosa. In normal nasal mucosa, UCN and CRF receptors were restricted to the vascular endothelium of submucosal cavernous sinusoids where faint staining was found. However, in allergic nasal mucosa, UCN was expressed in small vessels distributed in lamina propria and the vascular endothelium of cavernous sinusoid located in submucosa. Many scattered positive cells were also found in allergic nasal mucosa, probably UCN-positive leukocytes. CRF receptors were also localized in the vascular endothelium of small vessels and cavernous sinusoid. CONCLUSIONS: These results indicate that UCN may play a role in the regulation of vascular swelling in normal nasal mucosa. Moreover, in allergic nasal mucosa, increased expression levels of UCN and its receptors may contribute to increased mucosal swelling and vascular permeability, playing an important role in the pathogenesis of allergic rhinitis.

AB - OBJECTIVES: Urocortin (UCN) is a member of the corticotropin releasing factor (CRF) neuropeptide family. UCN act as locally expressed proinflammatory factor and induce mast cell degranulation, cytokine secretion, and trigger vascular permeability, which are mediated by CRF receptors in peripheral tissues. Considering its functional roles, UCN and its receptors may play a role in the pathogenesis of allergic nasal mucosa. Therefore, we investigated the expression profile and distribution of UCN and CRF receptors in normal and allergic nasal mucosa. METHODS: Reverse transcriptase-polymerase chain reaction, immunohistochemistry, and Western blotting were applied to the normal and allergic nasal mucosa. RESULTS: The expression levels of UCN and CRF receptors were increased in allergic nasal mucosa in comparison with normal nasal mucosa. In normal nasal mucosa, UCN and CRF receptors were restricted to the vascular endothelium of submucosal cavernous sinusoids where faint staining was found. However, in allergic nasal mucosa, UCN was expressed in small vessels distributed in lamina propria and the vascular endothelium of cavernous sinusoid located in submucosa. Many scattered positive cells were also found in allergic nasal mucosa, probably UCN-positive leukocytes. CRF receptors were also localized in the vascular endothelium of small vessels and cavernous sinusoid. CONCLUSIONS: These results indicate that UCN may play a role in the regulation of vascular swelling in normal nasal mucosa. Moreover, in allergic nasal mucosa, increased expression levels of UCN and its receptors may contribute to increased mucosal swelling and vascular permeability, playing an important role in the pathogenesis of allergic rhinitis.

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