Overexpression of a pepper ascorbate peroxidase-like 1 gene in tobacco plants enhances tolerance to oxidative stress and pathogens

Sujon Sarowar, Nam Kim Eui, Jin Kim Young, Han Ok Sung, Ki Deok Kim, Kook Hwang Byung, Jeong Sheop Shin

Research output: Contribution to journalArticle

69 Citations (Scopus)

Abstract

In order to determine the role of ascorbate peroxidase, an antioxidant enzyme, in the cellular responses to oxidative stress and pathogens, transgenic tobacco plants were generated, using the Capsicum annuum ascorbate peroxidase-like 1 gene (CAPOA1), under the control of the CaMV 35S promoter. High levels of CAPOA1 gene expression were observed in the transgenic plants, with a 2-fold increase in total peroxidase activity. The constitutive expression of CAPOA1 in the tobacco exhibited no morphological abnormalities, while significantly increased growth was observed in transgenic plants, as compared to control plants. The CAPOA1-overexpressed plants exhibited increased tolerance to methyl viologen-mediated oxidative stress, and also enhanced resistance to the oomycete pathogen, Phytophthora nicotianae. However, the transgenic plants were not found to be resistant to the bacterial pathogen, Pseudomonas syringae pv. tabaci, but were weakly resistant to Ralstonia solanacearum. Our results suggested that the overproduction of ascorbate peroxidase increased peroxidase activity that enhances active oxygen scavenging system, leading to oxidative stress tolerance and oomycete pathogen resistance.

Original languageEnglish
Pages (from-to)55-63
Number of pages9
JournalPlant Science
Volume169
Issue number1
DOIs
Publication statusPublished - 2005 Jul 1

Fingerprint

Ascorbate Peroxidases
Oxidative stress
Tobacco
Pathogens
ascorbate peroxidase
pepper
Capsicum
Oxidative Stress
tobacco
oxidative stress
Genes
Genetically Modified Plants
Capsicum annuum
pathogens
Oomycetes
transgenic plants
genes
Peroxidase
peroxidase
Ralstonia solanacearum

Keywords

  • Ascorbate peroxidase
  • Disease resistance
  • Oxidative stress tolerance
  • Reactive oxygen species
  • Transgenic tobacco plants

ASJC Scopus subject areas

  • Plant Science
  • Biochemistry
  • Biotechnology

Cite this

Overexpression of a pepper ascorbate peroxidase-like 1 gene in tobacco plants enhances tolerance to oxidative stress and pathogens. / Sarowar, Sujon; Eui, Nam Kim; Young, Jin Kim; Sung, Han Ok; Kim, Ki Deok; Byung, Kook Hwang; Shin, Jeong Sheop.

In: Plant Science, Vol. 169, No. 1, 01.07.2005, p. 55-63.

Research output: Contribution to journalArticle

Sarowar, Sujon ; Eui, Nam Kim ; Young, Jin Kim ; Sung, Han Ok ; Kim, Ki Deok ; Byung, Kook Hwang ; Shin, Jeong Sheop. / Overexpression of a pepper ascorbate peroxidase-like 1 gene in tobacco plants enhances tolerance to oxidative stress and pathogens. In: Plant Science. 2005 ; Vol. 169, No. 1. pp. 55-63.
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